Characterization of forage gemlplasm is an essential prerequisite for use of forage geneticrnresources. Fifty accessions of TrifoliulIl steudlleri Schweinf. were studied for 14rnmorphological quantitative characters, 9 morphological qualitative characters and 6rnagronomic characters. Spatial distribution of collection sites of accessions was checkedrnusing DIY A-GIS. Cluster analysis grouped the accessions into six groups, where the sixthrngroup had only one member, accession number 9452. A scatter plot made from the first andrnsecond factors of the factor analysis, which explains 33% of the total variation, and also thernisozyme analysis indicated this accession to be an outlier. A high correlation was observedrnbetween characters such as days to 50% flowering and 75% maturity, and leaf width andrnstipule length. Accession number 9452 was found to show a higher value of Mahalanobisrndistance, showing this accession is distantly related to the others. Taking eight agronomicrncharacters, accessions were grouped into three groups of high, medium and lowrnproductivity. The percentage frequency of phenotypic classes of the accessions for thernregions and altitude group and respective chi-square values for eight qualitative charactersrnwere calculated. The overall diversity index was calculated to be 0.40 Â± 0.07. Higherrnheritability values were observed for most of the quantitative morphological charactersrnexcept for stem thickness and flower width. Four enzyme systems, Peroxidase, Acidrnphosphatase, a-Esterase and p-Esterase, were used in the isozyme study, indicating thernpercentage of polymorphic loci ranges from 64.7 to 47.1. The isozyme clustering was foundrnto match with the morphological clustering.rnKey words: Characterization, TrifoliulII steudlleri, cluster analysis, factorrnanalysis, isozyme analysis, heritability, polymorphic loci, GIS