Molecular Epidemiology Of Hiv -1 Infection In A Cohort Of Ethiopian Factory Workers

In this study, to investigate the molecular epidemiology of the HIV-I virus in Ethiopia, thernHIV -I gp 120 V3 loop and flanking nucleotides were amplified from viruses isolated frolllrnthe plasma samples of23 I-IIV positive participants in the ongoing EHNRI-ENARP cohortrnproject in Akaki and Wonji factories. A nested PCR DNA amplification protocol yieldedrncorrect size products, as determined by agarose gel electrophoresis in 61 out of 75rn(81 %)plasma samples collected between 1996-1999. Out of these 61 samples, 53 (87%)rnwere sequenced successfully and analysed. Phylogenetic analysis determined that allrn V3 sequences are of the HIV-1 subtype C, thernmost predominant in Ethiopia. Also, the V3 sequences derived showed lower intra-personrnmutation frequencies than inter-person mutation fi•equencies. In addition, it could berndemonstrated that the Wonji and Akaki epidemics have a common origin and do notrnoriginate fi'om separate introductions. Finally, the proportions of non-synonymous (dn)rnversus synonymous (ds) mutations are variable per subject and are not dependent on CD4 counts or viral load.

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