The Biological Phenotypes(nsisi) Of Hiv-1 Isolates Obtained From Ethiopian Aids Patients Visiting St. Pauls Hospital In Addis Ababa.

The aim of thi.s study ,vas to assess the frequencies of syncytlum~tnducing and non~rnsyncytium-inducing HIY -, subtype C isolate. obtained from Ethiopian AIDS patient •.rnForty.eight hospitalized AIDS patients (CD4 + T cell count. < 100 cels/~ll) with WHOrnrevised conditions of stagtng system criteria for HIV"'1 i.nfection and disease Hsted inrnstage III and IY were used. The biological phenotype. of the viruses were determinedrnby MT5Z cocultivation ,21§say. Lymph.ocyte subsets were enumerated using coulterrncounter and FAC§can analysts. Vi.:ra .• load determination was done by nucleic addrnsequence ba.ed amplification assay (NASBA). HIY -, i.olates with syncytium-inducingrnphenotype were detected only in 3/48(6%) of AIDS patient •. Lower distribution ofrnabsolute CD4+ cell counts and CD4/CD8 r3t[0 were determined in 51 carriers asrncompared to N§l carders (P=O.04 and 0.03, respectively). However, no difference inrnviral load w.s observed between SI and NSI patients (P>o.OS). SI patients had higherrndi.tribution of irnrnune.complexed dissociated HIY- p24 levels than N§I patientsrn(P=o.oZ). Both patients with SI and N§I variant. had comparable CDS+ T cell countsrn(P=o.~). This finding suggests that the prevalence of 51 viruses among Ethiopian CornSIJIbtype AIDS patients was .ignificantly lower than among AIDS patients infectedrnwith other subtypes.

Get Full Work

Report copyright infringement or plagiarism