Background: Post operative wound infection is hospital acquired infection and it is the majorrncause of morbidity and mortality throughout the world especially in developing countries. Drugrnresistance is the challenge for controlling such bacteria now days. Assessing the prevalentrnbacteria and testing their antibiotic susceptibility helps to provide effective therapies, developrnrational prescription writing and make policy decisions.rnObjective: To determine the prevalence of multidrug resistant bacteria in postoperative woundrninfection.rnMethod: A cross sectional study was conducted from March 30 to August 28/2015 at TikurrnAnbessa Specialized Hospital, Addis Ababa, Ethiopia. A total of 197 study participants who hadrnbeen diagnosed for post operative wound infection during the study period were included. Allrncollected pus samples were cultured on Blood, MacConkey and Mannitol salt agar. All culturernpositive samples were characterized by gram stain and standard biochemical tests. Bacterialrnantimicrobial susceptibility test was performed on Muller-Hinton agar using Kirby-Bauerrnmethod.rnResults: The overall prevalence was 75.6% (n=149/197) and the predominant bacteria isolatedrnwere S. aureus33.3% (n=56/168), E. coli 14.3% (n=24/168 and Coagulase negativernStaphylococci 11.3% (n=19/168). Double infections 11.4% (n=17/149) were seen in which S.rnaureus and Pseudomonas species showed common association 23.5% (n=4/17). Multidrugrnresistances were recorded in 65.5% (n=110/168) of all bacterial isolates. Gram positive andrnGram negative isolates showed 55.3% (n=42/76) and 73.9% (n=68/92) multiple drug resistancernrespectively. Among the antibiotics tested amoxicillin (93.5%), Ceftriaxone (85.3%), Penicillinrn(84.5%) and Cefotaxime (82.7%) showed high level of resistance. Clindamycin (7.9%) andrnAmikacin (1.1%) showed high sensitivity for Gram Positive and Gram negative respectivelyrnConclusion: The choice of drugs in the treatment of bacterial isolates from postoperative woundrninfections is quite narrow today due to the wide scale resistance of pathogens to common drugs.rnRational use of antibiotics and a regular monitoring of antimicrobial resistance patterns in post-rnoperative wound infections are essential and mandatory to prevent further emergence and spreadrnof anti-microbial resistance among bacterial pathogens.rnKey terms: postoperative wound infections, Multidrug resistance bacteria, Tikur AnbessarnSpecialized Hospital, Addis Ababa, Ethiopia