Background: Cervical cancer is a preventable disease affecting an estimated 530,000 women each year andrnleading to nearly 275,000 deaths. Human papillomavirus (HPV) has been recognized as an important cause ofrncervical cancer and it is implicated in 99.7% of cervical squamous cell cancer cases in the world. It isrnrecognized as the third most common type of cancer in women worldwide and the second most prevalent cancerrntype and cause of cancer-related mortality in women in developing countries. In Ethiopia, every year 7095rnwomen diagnosed with cervical cancer and 4732 die from the disease. Very low screening practice andrninadequate screening coverage in the country makes cervical cancer as one of the major public health concern inrnEthiopia. There is also insufficient data on the prevalence of HR-HPV and cervical cytology abnormalities as arnnationwide basis.rnObjective: To assess the prevalence and genotype distribution of High Risk Human Papilloma Virus andrnCervical Cytology abnormalities among women attending selected Obstetrics and Gynecology clinics, of AddisrnAbaba, EthiopiarnMethod: Institutional based cross sectional study design was used in three selected Obstetrics and GynecologyrnClinics, Addis Ababa; from 15th June to 10th October 2015. Cervical samples were collected from the os of therncervix using Abbott cervi-cyt collection material for HR HPV DNA and cyto-brush for Pap smear screening. ArnStructured Interview based questionnaire was administered to assess the associated risk factors. A total of 366rnparticipants were enrolled based on the set inclusion criteria. High Risk HPV DNA was analyzed using AbbottrnReal Time PCR and cervical cytology screening using conventional Pap smear techniques. Data entry wasrnperformed using Epi-data version 3.1 and data analysis was performed by using STATA version 11.0.rnResult: The overall HR HPV prevalence was 13.7% (50/366), with 76% (38/50) of Other HR HPV genotypes.rnAbnormal cytology was observed in 13.1% (48/366) with 81.3%, 12.5%, and 6.3%, are LSIL, ASCUS andrnHSIL respectively. HR HPV DNA PCR and Conventional Pap smear cytology screening methods showedrnoverall agreement of 78% with kappa value of 0.12, 95% CI (0.00-0.243).rnConclusion and RecommendationrnIn this study, Non-16/18 genotypes contributed the largest proportion of the overall HR HPV. The highestrnfrequency of HR HPV positives was women without cervical cytology abnormality. The HR HPV with Paprnsmear co-screening in women whose age is >30 shall be in place. Further evaluation between the two screeningrnmethods against a perfect reference method shall be performed.rnKey WordsrnHigh Risk Human Papilloma Virus, Cervical Cytology, Obstetrics and Gynecology, Prevalence,rndistribution, PCR, LSIL, ASCUS, HSIL