Background: Urinary tract infection (UTI) is a very common infection both in the communityrnand hospital patients. Development of drug resistance by bacteriuria is a growing problem.rnAccurate identification of bacteria implicated causing UTIs and determining their drugrnsusceptibility pattern is critical for efficient management of patients with UTIs; that hasrnsignificant clinical and financial benefits, via reduction of mortality rates and overallrnhospitalization costs. The aim of this study was to determine the spectrum of bacteria implicatedrnin causing urinary tract infections and their drug susceptibility profile.rnMethodology: The present study was a single institutional cross-sectional study carried out atrnArsho Advanced Medical laboratory, Addis Ababa, Ethiopia from March to July 2016. Clean-rncatch midstream urine was collected from study participants and was inoculated onto primaryrnisolation culture media with calibrated loop. All plates were incubated at 37 0C for 18-24 hoursrnand the number of colonies was counted. Species identification and antibiotic susceptibilityrntesting of bacteria were determined with the automated VITEK 2 compact system.rnResult: Out of 712 urine samples processed, 256 (36%) yielded significant bacteriuria of whichrn208 (81.25%) were obtained from female study participants and 48 (18.75%) were from malernstudy participants. Cases of 75 % UTIs were recorded among age group of 15-64 years. Of thern256 bacterial isolates recovered, Escherichia coli, Klebsiella pneumoniae, Staphylococcusrnhaemolyticus and Enterococcus faecalis were the dominant bacterial isolates. Ampicillin (21.7%)rnand trimethoprim/sulfamethoxazole (33.7%) were the least effective drugs against gram negativernbacteria while piperacillin/tazobactam (82.3%) was the most effective drug. Erythromycinrn(17.8%) was the least effective drug against gram positive bacteria and daptomycin (98.1%) wasrnthe most active drugs. The overall resistance to two and more antibacterial drugs was highrn(75.4% and 91.3% for gram negative and positive respectively).rnConclusion: Isolation of many unpredictable bacteria, urinary tract infection rate of 36%, and anrnoverall higher resistance rate to two and more antibacterial (75.4% and 91.3% for gram negativernand gram positive bacteria respectively) were documented. These highlight the need forrnnationwide study on the spectrum and drug sensitivity pattern bacterial uropathogens.rnKey words: Antibiotics Resistance, Bacterial Profile, AAML, Urinary Tract Infection, VITEK 2rnCompact.