Background: Urinary tract infection (UTI) is considered as the most commonrnbacterial infectious disease seen among the pediatric patients.rnObjective:rnThis study was carried out in order to determine the antimicrobialrnSusceptibility Profile of Bacterial Uropathogens Isolates from Pediatric Patients at Yekatit 12rnHospital Medical CollegernMaterials and Methods Clean voided mid-stream urine specimens were obtained fromrnpatients in sterile universal bottles. Urine collected from each patient was inoculated ontornCLED and blood agar plats using calibrated inoculating loop with a capacity of 0.001ml,rnInoculated plates were incubated for 24- 48 hours at 37Â°C at inverted position aerobically.rnBacterial isolates were characterized/ indented by gram stain and by using an array ofrnstandard routine biochemical test. Antimicrobial susceptibility test was carried out by usingrnthe Kirby Bauer disc diffusion.rnResult: The data was analyzed by using SPSS, version 17. Descriptive statistics wasrncomputed for most of the study variables and Frequency distribution tables were used torndescribe the findings. In this study a total of 384 pediatric patients (199 males and 185rnfemales) aged between 0 years to 15 years from whom urine sample were collected werernenrolled. Of these patients, 61 (15.9%) had significant bacteriuria. Of the 185 females, 36rn(19.5%) have positive cultures while 25 (12.6%) of the 199 males had significant bacteriuriarnand the largest number of study subjects were below age 3 years and the largest positivernculture was obtained from this age group, accounting 35 (57.4%.) out of 61 positive culture.rnBacterial species belonging to six generarnwere isolated and identified from 61 positiverncultures and the genera were Escherichiai, Klebsiella, Staphylococcus, Proteus, Acnitobacterrnand Entrococcus. and E. coli was isolated in 28 cases (28/61, 49.5 %), followed by Klebsillarnspp. in 17 cases (17/61, 27.9%), Staphylococcus spp. in 5 patients (5/61, 8.2%.) ( S. aureus inrnone and coagulase negative staphylococci in 4 case), Entrococcus in 7 case (7/61, 11 5%),rnProteus spp. in 3 cases (3/61, 4.9%) ) and Acenitobacte in one case (1/61,1.6%). Of bacterialrnisolates E. coli was found out to be the most common pathogen followed by Klebsiella spp..rnFurthermore E. coli and klebsiella spp. were the most common pathogen in female patientsrnaccounting 71. 4% and 64.7% respectively. percentage resistance of Klebsilla spp was muchrnhigher when compared to E.coli. Eighty eight percent of Klebsiell spp. were resistant torncefotaxim, ceftazidim, trimetroprimsulfamethoxazole and cefuroxime. Acentobacter spp. wasrn100% resistances to gentamicin, trimetroprimsulfamethoxazole , agumentin, and nalidixicrnacid . But they were 100% susceptible to ciprofloxacin, cefuroxime , norfloxacine,rncefotaxim,chloramphenicol and ceftazidim . On the other hand, proteus spp. were 100%rnsensitive to all drugs except nitrofurantion. Species of Entrocuccus were resistance of 71.4%rnto chloramphenicol and 85.7% to both trimetroprimsulfamethoxazole and Erythromycin. S.rnauruesrnwasrn100%rnsusceptiblerntornciprofloxacin,rncefuroxime,rngentamicin,rntrimetroprimsulfamethoxazole, chloramphenicol, Clindamycine and Ceftriaxone whilerncoagulase negative staphylococci were resistance to all of the above drugs exceptrnClindamycine and gentamicin . Multidrug resistance to two or more drugs was observed inrn73.7% of bacterial isolates.rnConclusion: This study determines the antimicrobial Susceptibility Profile of BacterialrnUropathogen Isolates from Pediatric Patients and highlighted the majorrnbacterial uro-rnpathogens involved in UTI up to my knowledge for the first time in the country. Furthermore,rnspecies bacterial pathogens and their frequency was consistent with the usually reportedrnpattern, with E. coli being the most common organism isolated followed by Klebsiella. Spp.rnIn this study Majority of gram negative species were susceptible to ciprofloxacin,rnnorfloxacine and nitrofurantion (exceptrnproteus spp) and gram positive species werernsusceptible to ciprofloxacin Clindamycine and gentamicin so this drugs can be used to treatrnUTI in children however Most of bacterial isolates were multidrug resistant and it is thereforernsuggested that appropriate antimicrobials should be administered to reduce the risk of multirndrug resistant organisms developing and avert ineffectiveness of antibiotics