Prevalence And Antimicrobial Susceptibility Of Entrococcus Species Isolated From Different Clinical Samples At Tikur Anbesa Hospital Addis Ababa Ethiopia

Background: Enterococci which are part of the normal intestinal flora are opportunistic humanrnpathogens. The two most important species, E.faecalis and E.faecium are among the leadingrncauses of nosocomial infections that may cause severe infections including endocarditis, urinaryrntract infection, septicemia and wound infections which are often difficult to treat. Theirrnincreasing importance is largely due to their resistance to antimicrobials. So the aim this study isrnto determine the prevalence and antimicrobial pattern of Entrococcus spp.rnObjective: To determine the prevalence and antimicrobial susceptibility of Entrococcus speciesrnisolated from different clinical samples at Tikur Anbesa Specialized Hospital Addis Ababa,rnEthiopia .rnMethod: A cross sectional study was conducted from April to May 2016 on patients visitingrnTikur Anbesa Specialized Hospital. A total of 422 patients were included in the study. Clinicalrnsamples(blood, urine, pus, bodyfluid and csf) was inoculated onto blood agar and mackonkyrnculture media .The isolates were identified by cultural characteristics, Gram’s stain, catalaserntest, growth in 6.5% NaCl broth, bilesculin test, PYR test. Drug susceptibility pattern of thernisolates were determined using the Kirby Bauer disc diffusion method. Confirmation ofrnvancomycin susceptibility was done by the Epsilometer test to determine the MinimumrnInhibitory Concentration. Results obtained from the study was entered to excel spreadsheet andrntransport to and analyzed using SPSS version 20 to test for statistical associations. P values lessrnthan 0.05 were considered as significant.rnResult:From a total of 422 sample processed 15 Entrococcus spp were isolated .Among thernisolate 53.3% were female and 46.7 %were male patients . In this studyLizolide were the drug ofrnchoice for Entrococcus spp 100% sensitive followed by Vancomycen 93.3% sensitive inrncontrast Ampcillen was highly resistance by 80% followed by Doxicyclin 73.3% resistance.rnConclusion: This study showed that the rate of isolated Enterococci was 3.5% and had variablerndegrees of resistance to the antibiotics, but all were sensitive to Linzolide by disc diffusionrnmethods so the presence of VREand multidrug resistant Enterococci in our study should bernconsidered as an alarm for serious Enterococcal infections .rnKey words: Entrococcus, Nosocomial infection, clinical samples , Antibiotic susceptibility

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