Comparison Of Diagnostic Performance Of Mini Parasep Sf Faecal Parasite Concentrator Kato-katz Thick Smear And Mcmaster Thechniques For The Diagnosis Of Intestinal Parasitic Infections Among Wosha Soyama Primary School Childr

Abstract:-Background: Intestinal parasitic infections are responsible for considerable morbidityrnand mortality, especially among school-aged children. The diagnosis commonly relies on therndetection of trophozoite, cyst, oocyst, parasites egg, or larvae in stool. However, infections oftenrnescape diagnosis due to inefficiency of diagnostic methods among others (like, inappropriaternsampling and inadequate skill of laboratory personnel). So, the aim of this study was to evaluaternor compare the diagnostic performance or operational characteristics of three different diagnosticrntechniques for the determination of the prevalence and intensity of intestinal parasitic infections inrnorder to explore a better diagnostic method in the study area. Method: Institution based crosssectionalrnstudy was conducted in Wosha Soyama primary School, Wondo Genet area. Studentsrnwere selected by systematic random sampling and stool samples were collected. Samples werernscreened for the detection and intensity of intestinal parasitic infection using Mini parasep ® SFrnfaecal parasite concentrator, Kato-Katz thick smear and McMaster egg counting technique. Datarnwere entered in to Excel and analyzed with SPSS version 22 and Stata version 13.rnRESULT: - Overall 86.1% (328/381) of samples were found positive for intestinal parasiticrninfection. Poly-parasitism were found in 54.1% (206/381) of the stool samples examined. Thernprevalence of intestinal parasites using the Mini parasep ® SF faecal parasite concentrator, Kato-rnKatz and McMaster were 77.7% (296/381), 68.8% (262/381) and 47.5% (181/381), respectively.rnThe sensitivity, specificity, positive and negative predictive value of Mini parasep ® SF faecalrnparasite concentrator were 90.2%, 100%, 72.7% and 100%, for Kato-Katz thick smear 80.0%,rn100%, 64% and 100% and for McMaster 55.2%, 100%, 57.6% and 100%, respectively.rnCONCLUSION: Mini parasep ® SF faecal parasite concentrator detects many helminthsrnincluding cysts of protozoan parasites than the two methods. It had low egg counting abilityrncompared to the two methods, except higher mean egg count for Hymenolopis nana and Hookrnworm than Kato -Katz thick smear. Kato- Katz also showed better performance than McMasterrnexcept for Hookworm and Hymenolopis nana. McMaster showed better performance forrnHookworm than both methods. In addition it had better performance than Kato- Katz forrnHymenolopis nana. Kato-Katz also had more sensitivity and negative predictive value thanrnMcMaster. Moreover, Kato Katz showed better performance in assessing intensity of infection forrnmost helminths detected than the two methods. Intestinal parasitic infections showed alarmingrnprevalence and intensity of infection in study area. Recommendation: For mapping priority areasrnfor control, monitoring and evaluation of programs impact, or for surveillance purpose andrnroutine diagnosis of intestinal parasites further evaluation of Mini parasep ® SF relative to otherrnmethods and strength health promoting activities in this area is recommended.rnKeywords: Diagnostic performance, Mini parasep® SF, Kato-Katz, McMaster, Intestinalrnparasites, school children, Ethiopia

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