Background: Brucellosis is a zoonotic disease usually acquired through direct contactrnwith the infected animals and consumption of contaminated milk and meat products.rnIn humans Brucellosis presents similar signs with other febrile diseases like Malaria,rntyphoid and other febrile conditions.rnObjective: In this study weaimed to determine Sero-prevalence of human brucellosisrnmalaria co-infection and risk factors among febrile patients visited Derayitu Healthrncenter and Kelewani Primary Hospital at Awra and Gulina district, Afar Region, EthiopiarnMethods: A cross Sectional study was conducted among febrile patients visitingrnDerayitu Health center and Kelewani Primary Hospital at Awra & Gulina District of Afarrnregion, Ethiopia from February to May 2019. After consent/assent obtained,rndemographic and clinical data were collected using structured questionnaire, 5mlrnvenous blood was collected with plain vacutainer tubs. Serum was separated andrnstored in refrigerator after thick and thin blood films were prepared. Rose Bengal platernTest (RBPT) was performed and those positive sera were confirmed using ELISA.rnGiemsa stained thick and thin blood films, were made to look for the presence ofrnhemo-parasites. Data were entered in Epi Data3.1 and exported to Stata 14 to analyzernprevalence and potential risk factors at 95% confidence interval < 0.05 was consideredrnas statistically significantrnResults: A total 444 febrile individuals (females constituting of 61.1%), age rangedrnfrom 2 to 83 years (mean = 26.1, SD = Â±11.8) were participated in this study. Among allrn(444) individuals tested, 31.5 % (140/444) were found reactive by Rose Bengal platernTest and only half of the reactive (70/140) were found reactive again by EnzymernLinked Immuno sorbent assay. The sero-prevalence of Brucellainfection based on therntwo tests was 15.8 % (70/444). Being males (23.3%, X2= 13.05; p