Background: Drugs available to treat diseases are not without problems and the same isrntrue for drugs of Tuberculosis and Human Immunodeficiency Virus infections. Drugrninduced hepatotoxicity is the major serious adverse drug reactions encountered during therntreatment of these diseases. Anti tuberculosis drugs are very well known causes of druginducedrnrnliver injury. Ethiopia is highly afflicted by the TB pandemic and is ranked 14rnrnamong the high Tuberculosis burden countries worldwide implying that large amount ofrndrugs are used annually to treat these diseases which are capable of inducing liver injury.rnAnemia is also another aberration in such chronic diseases that may be induced by theserndrugs. Thus, this study focused on assessment of drug induced hepatotoxicity and anemiarnamong Tuberculosis infected children. rnMethodology:- Institutional based comparative cross-sectional study was conductedrnfrom May-November, 2019 at Maychew, Adigrat, Mekelle, and Axum general hospitals.rnA total of 188 participants (94 Tuberculosis infected children and 94 control groups)rnwere recruited and blood samples were taken for Liver function and hemoglobin test tornassess drug induced liver injury and anemia among the Tuberculosis infected children. rnResult: Among the participants, 54.3% male and 45.7% female TB infected children andrnthere was 58.5% male and 41.5% female in the control group. The mean age ofrnTuberculosis infected children was 9.46+(4.249), whereas the mean age of the controlrngroup was 8.35+(4.285) years. The mean serum enzymatic levels were elevated amongrnportion of TB infected children (i.e. Aspartate aminotransferase 59/94(62.7%), Alaninernaminotransferase 41/94(43.6%); and Total Bilirubin 37/94(39.4%)). Drug inducedrnhepatotoxicity 8/94(8.5%) and anemia 29/94(30.9%) were observed in tuberculosisrninfected children. In this study only 11/94(11.7%) of the control group were anemic. rnConclusion: ALT, AST and Bilirubin in this study were significantly increased amongrnTuberculosis infected children and TB-HIV co-infected children when compared torncontrol group and TB only infected children respectively. Whole blood hemoglobinrnconcentration were significantly decreased in Tuberculosis infected children comparedrnto control group. The Anti-Tuberculosis drugs seem to induce hepatotoxicity and anemiarnover a time of period.rnrnth