Introduction: Most of breast cancers are induced by abnormal levels of estrogen,rnprogesterone, and changes in lipid profiles. Cancer cells metabolize lipids unlike normalrncells and affected by steroid hormones. Estrogen or estradiol controls metabolism ofrnlipids and it regulates rate of progesterone metabolism. Progesterone regulates the effectrnof estradiol.Tamoxifen and armidex are drugs that block estrogen action on its receptorsrnor decrease estrogen levels. Although, they change progesterone and lipid concentrationsrnin breast cancer patients this may induce adverse effects like hyper /hypo estrogenicrnproperties and lipidimea and other diseases as secondary complications. Thus,rnassessment of steroid hormones and lipid profile levels are necessary for breast cancerrnpatients, but in Ethiopia, it has not yet been studied and used for treatment. rnrnObjective: - To assess the relationship between serum estradiol, progesterone and lipidrnprofiles level among breast cancer patients before and after hormonal therapy at TikurrnAnbessa Specialized Hospital. rnrnMethods: Cross-sectional study design was used to assess the relationships betweenrnserum estradiol, progesterone and lipid profile levels in breast cancer patients before andrnafter hormonal therapy by comparing with healthy controls. Convenient sampling methodrnwas used to select 120 participants. Immunochemistry analyzer was used to measurernsteroid hormone levels and lipids were measured by chemistry analyzer. Data analyzesrnwere done by independent sample t-test, Oneway ANOVA and Pearsonâ€˜s correlationrncoefficient. P-value < 0.05 was taken as statistically significant.rnrn Results: In breast cancer patients without medication significantly lower mean serumrnprogesterone, TC and HDL-C levels at (p0.05) were observed when compared with healthy controls.rnSignificantly higher mean serum TC, LDL-C, TG, HDL-C levels (p