Molecular Characterization And Drug Resistance Pattern Of Mycobacterium Tuberculosis Complex Among Confirmed Extrapulmonary Tuberculosis Cases In Addis Ababa Ethiopia

Background: Molecular characterization and drug-resistant of Mycobacterium tuberculosis is arnvaluable tool in understanding the pathogenesis, diagnosis treatment, and prevention ofrntuberculosis. However, there is limited information on molecular characterization, and drugresistantrnrnpatterns of Mycobacterium tuberculosis in patients with extrapulmonary tuberculosis inrnEthiopia. rnObjective: This study aimed to determine the molecular characteristic and drug resistancernpatterns of Mycobacterium tuberculosis complex among confirmed extrapulmonary tuberculosisrncases in Addis Ababa, Ethiopia rnMethods: A laboratory-based cross-sectional study was conducted among confirmedrnextrapulmonary tuberculosis cases in Addis Ababa, Ethiopia in a period between Novemberrn2019 and April 2020. Drug susceptibility test was performed using BACTEC-MGIT 960 and rnLine probe assay. Strain identification was performed using the Geno-Type MTBC andrnspoligotyping technique. Data were entered into the international spoligotyping database tornassess the spoligotype patterns of Mycobacterium tuberculosis. Data was analyzed by SPSSrnversion 23 and participants’ characteristics were presented by number and proportion. rnResults: Of 151 Mycobacterium tuberculosis complex isolates, 29 (19.2%) were resistant to atrnleast one drug, and 14 (9.3%) were identified as multidrug-resistant tuberculosis. Besides,rn21.4% (3/14) of isolates had pre-extensively drug-resistant tuberculosis. S315Tl (11.9% (18/151)rnwas the most common isoniazid mutation observed in the katG gene. The spoligotyping of thern151 isolates resulted in 41 different spoligotype patterns. The most abundantly represented SITsrnwere: SIT149 21.2% (33/151), SIT53 14.6% (22/151) and SIT26 9.6% (14/151). The mostrnpredominant lineage was T family 55.0% (83/151), Central Asia Strain 19.2% (29/151) andrnHaarlem consisted of 8.0% (12/151). Also, 5 (3.3%) isolates had no matching with the database. rnConclusion: The present study showed a high proportion of multidrug-resistant tuberculosis andrnpre-extensively drug-resistant tuberculosis among EPTB patients. The strain was mostly groupedrninto SIT149, SIT53, and SIT26. A large scale is required to define the molecular characteristicsrnand drug resistance of Mycobacterium tuberculosis in extrapulmonary tuberculosis patients.

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