Prevalence Of Extended-spectrum B-lactemase And Carbapenemase Producing Enterobacteriaceae Among Adult Patients Complaining Uti At South West Shoa Lemman Hospital And Kersa Malimma Woreda Health Facility Ethiopia

Background: Infection caused by Extended-spectrum beta-lactamase- (ESBLs) and Carbapenemasernproducing Enterobacteriaceae (CPE) are serious problems of the world. Enterobacteriaceae are the mostrnimportant producers of ESBLs and Carbapenemase enzymes. rnObjective: To determine the magnitude of Extended -spectrum B-Lactemase and Carbapenemasernproducing Enterobacteriaceae patients complaining Urinary tract infection(UTI) at South West shoa, rnlemman Hospital and kersa malima woreda health facility, Ethiopia rnMethods: Across-sectional study was conducted from a total of278 symptomatic UTI suspected adultrnpatients fromSeptember1/2020 to December 30/ 2021. A convenient sampling technique was used tornenrolled patients in this study .Socio demographic data and other information was collected usingrnrequisition form. Midstream urine samples were collected from each patient and inoculated ontornMacconkey and Sheep blood agar media. Inoculated plates were incubated at 37rnC for 18-24 hoursrnaerobically. The identification of Enterobacteriaceae and gram positive bacteria were carried on based onrnnumber of colonies, type of colonies, morphological appearance, and Gram reaction after growth onrnculture media. Carbapenemase and ESBLs producing Enterobacteriaceae were performed by usingrncombination disk. Finally, data were analyzed using analytical binary regration SPSS version 24. rnResults. The overall prevalence of Enterobacteriaceae isolates from urine cultures was 16.9 %(rn47/278).The highest isolated bacteria were Escherichia coli 78.7% (37/47) and Klebsiella pneumoniaernwere 8.5% (4/47). Most of them 51.1% (24/47) developed multidrug resistance (MDR≥3 drugs) to mostrncommonly used antibiotics. Prevalence of ESBL producing and Carbapenems resistancernEnterobacteriaceae was 44.7% and 0% respectively. The overall prevalence of ESBLs was 44.7%rn(95%CI; OR=2.68,P=O.582) among all isolates and 18/21 (85.7%) of ESBLs isolates were identifiedrnfrom females and 42.9 % (9/21) isolates were also indicated from patients with age group from 25-34rnyears. rnConclusion. ESBL β-lactamases producing Enterobacteriaceae were highly susceptible tornCarbapenemase, piperacillin/tazobactam and more susceptible Nitrofurantoin. Consequently, the argentrnof ESBLs producing Enterobacteriaceae requires continuous testing& reviewing of antimicrobialrnstrategy in hospitals and health facility in this area are increased. All carbapenems strains are sensitivern(susceptible) for all Enterobacteriaceae isolates.

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