Background: Catheter associated urinary tract infections (CA-UTIs) are common cause ofrnhospital acquired infections. The emergence of Vancomycin-resistant Enterococcus (VRE),rnposes a significant problem in the management of CA-UTI. There is scarcity of information onrnthe prevalence and drug resistance pattern of VRE among catheterized patients in the currentrnstudy setting.rnObjective: To determine the magnitude & prevalence of CA-UTI and CA-VRE in catheterized rnpatients and to assess the knowledge and practice of health professionals on CA-UTI.rnMethods: A hospital based cross-sectional study was conducted on 270 catheterized patients rnadmitted to Yekatit 12 Hospital Medical College from December 2020 to June 2021. A total ofrn121 health professionals were also assessed for their KAP on CA-UTI. Urine samples wererncollected and inoculated on to blood and MacConkey agars, incubated at 35-37Â°C for 24 hoursrnaerobically. Phenotypic identification was carried out by biochemical tests. Vancomycinrnresistance for Entrococcus was performed by E-test. Data on level of knowledge and practice onrnCA-UTI was collected from health professionals using questionnaire and analysed by usingrnSPSS version 20.P-value < 0.05 was used as statistically significant.rnResult: CA-UTI was detected in 31.9% of the study participants. E.coli was the most prevalentrnisolate (9.3%) followed by Enterococci (7.4%).The Enterococcus isolates showed highestrnresistance for penicillin (95%) followed by Doxycycline (55%) and erythromycin(50%). Inrncontrast, lower resistance was documented for Ciprofloxacin (25%), Meropenem (20%) andrnVancomycin (20%).The prevalence of CA-VRE among the Enterococcus isolates was 20%.rnAmong health professionals participated in the study, 94.2% had knowledge on CA-UTI. Onlyrn34.7% had awareness on CA-VRE and 19.8% had knowledge on bacterial biofilm formation. rnConclusion: The overall prevalence of CA-UTI and CA-VRE was 31.9% and 20% respectively.rnMedical ward, intermittent catheterization and previous UTI have strong association with CAUTI,rn