Background: Diabetes mellitus is a non-communicable disease that has a high prevalencernworldwide. Altered level of many hematological and biochemical parameters have beenrnobserved in patients with diabetes. Although type Ó€Ó€ diabetes mellites alters hematological andrnbiochemical parameters, so far, very little work has been done in Ethiopia linking hematologicalrnparameters and biochemical markers with type Ó€Ó€ diabetes mellites patients. rnObjective: To assess alteration of hematological and biochemical parameters in type Ó€Ó€ diabetesrnmellitus patients attending Aksum St. Marry Hospital, Aksum, Northern Ethiopia, fromrnDecember 2019-April 2020 rnMethod: comparative cross-sectional study was conducted from December 2019 to April 2020 at rnAksum St. Marry Hospital, Aksum, Northern Ethiopia. A total of 168 participants were enrolledrnusing convenient sampling method. Socio-demographic, behavioral and physical measurementrndata were collected. Complete blood count was performed by sysmex KX-21N hematologyrnanalyzer. The biochemical parameters were analyzed by using BE200. Data was analyzed usingrnSPSS 23. Student independent t-test and personâ€™s correlation statistical method employed tornanalyze data. P values < 0.05 was regarded as statistically significant at 95% confidence level. rnResult: Among the hematological parameters WBC count, neutrophil count, lymphocytes count,rnplatelet count, MPV significantly increased. Similarly, RBC count, hemoglobin and PCVrnshowed significant decrease in T2DM patients compared to controls. With regard to biochemicalrnparameters ALT, AST, ALP, Creatinine and urea showed significantly increasing in T2DMrnpatients when compared to controls. Regarding correlation, WBC with BMI and MCHC withrnFBS had positive correlations. rnConclusion: There is alteration in total WBC, neutrophil, lymphocyte, eosinophil, basophil,rnRBC, hemoglobin and PCV. Also, from biochemical parameters ALT, AST, ALP, urea andrncreatinine altered in diabetes patients compared with control group.