Background: Non-Fermenting Gram-Negative Bacilli are defined as strictly aerobic and nonsporernformingrngrouprnofrnbacteria.rnTheyrnarernintrinsicallyrnresistantrntornmanyrnantibiotics.rnGloballyrnthernrnburdenrnrnof multidrug resistance in Gram-negative bacilli represents a daily issue for thernmanagement of antimicrobial therapy in patients. rnObjective: The aim of this study was profiling and antimicrobial susceptible pattern of nonfermentativernrnGram- negative bacilli isolated from patients referred to Arsho Advanced Medicalrnlaboratory Addis Ababa, Ethiopia. rn Methods: A cross sectional study were conducted in Arsho advanced medical laboratory AddisrnAbaba, Ethiopia from January 2020 to August 2021 on 705 clinical samples. From this, isolatesrnwere detected from different clinical samples such as urine, body fluid, blood, CSF and otherrnsamples which was collected from patients. All the samples received in the microbiologyrnlaboratory were inoculated on blood agar, MacConkey agar and chocolate agar and incubated atrn37Â°C for 48hr. Non fermentative Gram-negative bacilli was isolated and identified from clinicalrnspecimens by standard procedure and antibiotic sensitivity test was performed by VITEC 2.rnGram reaction of the organisms was determined by Gram stain. rnResults: From the total of 705 various specimens, 137 (34.5%) were culture positive for NonfermentativernrnGram-negative bacteria, Among the isolated bacteria, the highest number wasrnAcinetobacter baumannii 26/137(3.7%). While Pseudomonas aeruginosa accounts 20/137 (2.8)rnfollowed by Proteus mirabilis 16/137 (2.3%). Among NFGNB P. aeruginosa (100%), and A.rnbaumanii (100%) were multi drug resistance. rnConclusion: Antimicrobial resistance and multi drug resistance was performed during the studyrnperiod was high, it is due to ordering antibiotic without knowing the right pathogen and alsornunlimited use of antibiotics.