Background: Measuring of Viral load in the plasma of patient is the most important indicator inrnresponse to Anti Retro viral treatment, and to monitoring regularly progress of the patient, byrnsuppressing the Viral Load to a threshold of < 1000 RNA copies/ml, which is used to definernsuppressed viral load, if it is confirmed in the laboratory. adherence will be addressed andrnfollowed to switch to second-line Anti Retro viral treatment. rnObjective: the aim of this study was to determine viral suppression rate and associated riskrnfactors among children tested for HIV viral load among patient‟s sample refereed to Ethiopianrnpublic health institute, Addis Ababa, Ethiopia. rnMethod: Across-sectional study was conducted. Data on socio-demographic factors includingrnAge, sex, test reason, treatment combination, adherence, treatment duration on Anti Retro viralrntreatment, WHO clinical staging, were extracted from the request, Viral suppression and failurernwas determined using the WHO definitions (viral suppression as viral load < 1000/ml) and theirrnassociated factors. Significant association between study variables and interpretation of data wasrndone using the adjusted odds ratio (AOR) and 95% confidence interval and P value 1000cop/Ml) more likelyrnhad viral suppression rate (AOR=0.261;95% CI= 0.94-0.723), children between Age 3-10,werern(AOR=17.76; 95% CI=1.623-194.19). rnConclusion The rate of un-suppressed viral load is High among children tested for HIV ViralrnLoad in Ethiopian Public Health institute and Children adhered to ART treatment have more rnlikely to be virally suppressed.