Background: Contaminated workings clothe and hand of health care workers can causernnosocomial infections. Staphylococcus aureus (S aureus) is the main causative agent ofrnnosocomial infection and it can be transmitted through working clothe of health care workersrnfrom patient to health care workers and from health care workers to patients at hospitals. Poorrnhand hygiene is the main way of spreading infectious organisms. rnObjective: To assess Staphylococcus aureus and MRSA contamination level of Working Clothes,rnHand carriage, and knowledge, attitude, and practice (KAP) among Health Care workersrn(HCWs) at Federal Police Hospital. rnMethods: Cross-sectional study was conducted at the Federal police hospital (FPH) fromrnNovember 2020-March 2021 by inclusion of 222 healthcare workers. 222 swab samples wererncollected from the working clothing of all participants and 53 hand swab samples were randomlyrncollected from 53 HCWs. Culturing, identification, and antimicrobial sensitivity tests werernperformed at the Federal Police Hospital microbiology laboratory using conventional standardrnmicrobiology tests. The positive and negative control organisms' S aureus and S epidermidisrnwere obtained from Ethiopian Public Health Institute (EPHI) microbiology department. Therndemographic data, knowledge, attitude, and practice of study participants were collected byrnproviding self-administered questioner and observational checklists. The data entry was done byrnMicrosoft Excel and chi-square data analysis done by using SPSS version 21 software. rnResults: Out of 222 study participants, 39(17.6%) were contaminated with staphylococcusrnbacteria. A total of 41 staphylococci were isolated from 35 working clothes,2 hands, and 2 werernisolated from both clothes and hands. From a total of 41 isolates, 18(43.9 %) S aureus, andrn23(56.1%) coagulase-negative Staphylococcus (CoNS) were identified. 12(67%) Methicillinrnsensitive S aureus (MSSA) and 6(33%) Methicillin-resistant S aureus (MRSA) were identifiedrnfrom a total of 18 S aureus isolates. About 86.9% of study participants have good knowledgernabout the use and handling of working clothes related to bacterial contamination. 60% of themrnshow a good attitude and 70.1% were practicing good on using and handling of working clothesrnrelated to the prevention of bacterial contamination. rnConclusion: In this study the contamination rate of working clothe and hand carriage of S rnaoreus was 6.8% and 5.6% respectively and 33% of the isolates were MRSA. Even though studyrnparticipants have good knowledge, attitude, and practice about working clothe and hand hygienerncontinuous awerness of staff is paramount to prevent MRSA in the hospital.