Molecular Characterization And Assessment Of Viral Tumorigenesis In Breast Cancer Among Women In Addis Ababa Ethiopia

In this study from Tikur Anbessa Specialized Hospital, 114 breast cancer patients diagnosedrnbetween 2012 and 2015 were enrolled. Estroge receptor (ER), Progesterone Receptor (PR), Ki-rn67 and Human Epidermal Growth factor (HER-2) receptor status were assessed usingrnimmunohistochemistry (IHC) from tissue microarrays (TMA). Fluorescence in situ hybridizationrn(FISH) and Gene Protein Assay (GPA) was used for assessment of gene amplification in allrnequivocal tumor samples and for confirmation in HER2-enriched cases. Androgen Receptorrn(AR) was assessed using IHC from TMA and BRCA1 was assessed using IHC from wholernsection. EBV, HCMV and HPV viral proteins or/and DNA were assessed using IHC or/andrnmultiplex qPCR. The 2013 St. Gallen international panel of expert’s recommendation forrnclassification of breast carcinoma based on IHC was applied to molecularly classify the tumors.rnInformation obtained also included age, tumor grade, histological type, and stage of disease.rnIn this study, the most common molecular subtypes was Luminal A (40%) followed by LuminalrnB (26%), TNBC (23%) and HER2-enriched (10%). ER were positive in 65% of all tumors andrn43% the participants were positive for PR. There was statistically significant variation in medianrnage at diagnosis between the different molecular subtypes (P

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