Introduction:-Hypertension is a chronic elevation of systemic arterial pressure above arncertain threshold value. It is a serious health problem worldwide that contributes tornmyocardial infarction, cerebrovascular accidents, and premature mortality. Medicinal plantsrnare used to treat different diseases in different parts of the world. There are number ofrnmedicinal plants in the management of hypertension. One such plant is Ruta chalpensis whichrnis used to treat different ailment in different parts of the world.rnObjective: - The objective of this study was aimed to screen the secondary metabolites and tornidentify whether the aerial part of crude extract of Ruta chalpensis alters blood pressure andrnits effect on reactive oxygen species.rnMaterials and methods: -The study was conducted on twelve guinea pigs to measure therneffect of Ruta chalpensis 70% ethanol extract by using invasive and organ bath methods.rnQualitative chemical screening involving color change was applied to see the phytochemicalsrnpresent in plant extract, and Spectrophotometric analysis by DPPH method has been used tornsee the antioxidant activity.rnResult:- The areal part of 70% ethanol extract of Ruta chalpensis was found positive for,rnmajor secondary metabolites, The IC50, value was computed to be 21.72mM of ascorbic acidrnand 58.20mM of 70% ethanol extract of areal part of Ruta chalpensis and an inhibition ofrn88.70% and 96.96% at 500ug/ml of the extract and the standard ascorbic acid respectively.rnAfter induction of aortic muscle contraction by high concentration of k+ the extract showed arnsignificant (p