Background:-Ocular infection is a major public health problem in developing countries.rnBacteria and fungi associated with external ocular infection are responsible for increasedrnincidence of morbidity and blindness worldwide.rnObjective:-The aim of this study was to determine the profile of bacterial and fungal pathogensrnfrom external ocular infection at St. Paul Hospital Millennium Medical College, Addis Ababa,rnEthiopia.rnMethodology:-A cross sectional study conducted using convenient sampling technique. A totalrnof 215 patients recruited from April to August, 2016. Conjunctival and eyelid margin swabs andrncorneal scraping were collected. Demographic data were collected using structuredrnquestionnaire. All Specimens were processed for microbiological analysis as per standardrnprocedures and antibiotic susceptibility test was done using disc diffusion technique. The datarnwas analyzed by using SPSS version 20 and appropriate statistical tools were used.rnResult: - A total of 215 patients were enrolled in this study. Of which 133(61.4 %) were culturernpositive. Of this 118(54.9%) and 24(11.2%) were bacterial and fungal pathogens respectively.rnThere were 9 (6.8%) bacterial and fungal co-infections. Majority were males 109 (50.7%). Thernmean age of the study participants was 42.34 (Sd. +20.55) and majority of participants age werernwithin the age range of 25-44 years 72(33.5%). Majority had conjunctivitis 87(40.5%) followedrnby blepharitis 48(22.3%). The predominant bacterial pathogen isolated were gram positivesrn88(74.6%). Of this S. aureus 32(27.1%) was the commonest isolate and K. pneumoniae 9(7.6%)rnwas the predominant from gram negative bacterial pathogens. Aspergillus species 14(58.3%)rnwere the most predominant fungal isolates. Gram positive cocci and gram negative rods werernhighly resistant for Penicillin 66/88(75%) and Ampicilin 20/27(81.5%) respectively. 71.2% ofrnMDR bacteria were isolated. There was statistically significant association between prevalencernof bacterial isolates and age variation (P=0.023) and trauma with fungi prevalence (P=0.002).rnConclusion:-The prevalence of bacterial and fungal pathogens was higher among external ocularrninfection. The drug resistance among ocular bacterial infection was also higher.rnKeywords: - External ocular infections, conjunctivitis, blepharitis, keratitis, dacryocystitis,rnsusceptibility