Background: Wound develops into an infected state when the balance between microorganismrnand the host shifts in favour of the micro-organism. Antimicrobial resistance occurs whenrnbacteria change in some way that reduces or eliminates the effectiveness of drugs.rnObjective: The main objective of this study was to isolate etiology of wound infections andrndetermine their antimicrobial susceptibility pattern.rnMethods: A cross-sectional study was conducted at ALERT Center from February to May 2017.rnSwabs from different types of wounds was taken and processed to isolate etiologic agents byrnusing standard microbiological techniques. Antimicrobial susceptibility tests were performed byrndisc diffusion technique as per the standard modified Kirby-Bauer method.rnResults: In this study 171 bacterial isolates were recovered from 188 specimens showing anrnisolation rate of 86.2%. The predominant bacteria isolated from the infected wounds werernStaphylococcus aureus 96 (51.1%) followed by Klebsiella pneumoniae 26 (15.2%), Escherichiarncoli 23(13.4%). Out of 162 positive samples 9(5.5%) were mixed infections. Staphylococcusrnaureus exhibited highest sensitivity against Clindamycin (95.8%), Gentamycin (94.8%),rnChloramphenicol (92.7%), Ciprofloxacillin (89.6%) and Cotrimoxazole (84%). Gram negativernisolates, E.coli, P.vulgaris, P.mirabilis, P.aeroginosa and Citrobacter showed the highestrnsensitivity against Amikacin (100 %). E.coli showed high resistance for Ampicilin (95.7%) andrnAugumentin (91.3%) where as P.vulgaris showed 100% resistance for Ampicilin and 90.9 % forrnTetracycline.rnConclusion: There was high prevalence of bacterial isolates in this study. S. aureus was thernpredominant isolate 96 (56.1%). Most of the isolates showed high resistance to commonly usedrnantimicrobials. The antimicrobial profile of drugs demonstrated that the commonly prescribedrndrugs against Gram positive bacteria (Penicillin, Tetracycline) and Gram-negative bacteriarn(Ampicillin and Tetracycline) as a single agent for empirical treatment of wound infectionsrnwould not cover the majority of wounds infections. Antimicrobial treatment should be based onrnthe result of culture and sensitivity.rnKeyword: wound infection, bacterial isolates, drug resistance pattern.