Background: Persons working in clinical diagnostic laboratories are exposed to many risks. The mostrnhazardous agent in the laboratory is a microorganism that is frequently associated withrnlaboratory infections and can be transmitted in variety of routes, especially by aerosols.rnMoreover, hospital and diagnostic laboratories are a challenging environment for the surroundingrncommunity. The more the laboratorians become aware of and adhere to recommended, science-basedrnsafety precautions, the lower the risk for laboratory acquired infections. The behavior patterns andrnattitude of individuals towards safety programs influence their involvement in laboratoryrnaccidents that put themselves and fellow workers at risk. Assessing laboratory biosafety practicesrnwill help the laboratories to identify factors that hindered laboratory personnel from practicingrngood laboratory biosafety measures and to determine the existing situation of the assessedrnlaboratories so that laboratory managers can plan towards implementation of biosafety practicesrnand hence safe working laboratory area will be created.rnObjective:The aim of this study was to assess biosafety practices among laboratory personnelrnworking in selected public hospitals in the East, West and S/West Shoa zones of Oromia region.rnMethods: Institution based cross-sectional study was conducted between December 2013 andrnMay 2014.The study was conducted among 45 studied laboratory personnel working in 5rnselected public hospital laboratories located in East, West and S/West Shoa Adama, Bishoftu,rnTulubolo, Ambo and St. Luke hospital laboratory). Datawas collected between April and Mayrn2014 using structured questionnaire. The analysis was done by SPSS version16 statisticalrnsoftware using one-way ANOVA. P-values less than 0.05 wereconsidered as statisticallyrnsignificant.rnResult:Out 0f 45 participantsgreater than 37 (84%) of studied personnel were within the agernrange of 18-44 years. Of which the majority 23(64%) of the studied laboratory personnelsâ€™ agernwere aggregated within the age distribution category of 25-34. Male to female ratio of thernstudied participants were 3:1 with a count of male participant 34 (75.6%), and females accountedrn11 (24.4%). Regarding the level of education, 23 (51.1%) of them had finished undergraduaternstudy on medical laboratory sciences (Bachelor of sciences) and the rest 21(46.7%) wererndiploma holders in laboratory science. The duration of work experience in laboratory service ofrnthe studied laboratory showed that greater than 33(75%) of the group had a work experience ofrngreater than 5years. This survey shown that the grand mean of the studied laboratory personnelrnfor the overall biosafety practices was found to be 36.44Â±7.635 with a mean score range of 52%-rn80%. Laboratory personnel B exceptionally scored the highest mean score of all 42.7(80%) andrnfall in upper moderate level which is close to high level. The mean score for laboratory D and Ernhad shown similar results which are a mean value of38.6 (70%)and they are closed to be labeledrnas moderate. The rest two laboratories (A and C) found to be low level with laboratory A scoredrnthe least mean value of all 29.7 (50%), while laboratory C scored a mean value of 34.5.rnConclusion: The overall biosafety practice of the studied hospital laboratories average score isrn36.44Â±7.63 from a total score value of 55. The percent range forall studied labs is 52%-80%.rnLaboratory B exceptionally scored the highest mean of all with a score of 80% as a result it is inrnthe upper moderate level category.