Antibiotic Resistance Patterns Of Gram Negative Fermentative And Non Bacilli Isolated From Patients Referred To Arsho Advanced Medical Laboratory Addis Ababa Ethiopia

Background: Gram negative bacilli are the most frequently isolated bacteria which recoveredrnfrom various clinical samples. Conventionally, these microorganisms subdivided into two majorrngroups’ fermentative and non-fermentative gram-negative bacteria. The aim of this study is torndetermine antibiotic resistance patterns of fermentative and non-fermentative gram negativernbacilli from various clinical samples in study area.rnMethod: A laboratory based cross-sectional study conducted at Arsho Advanced Medicalrnlaboratory,rnAddisrnAbaba,rnEthiopiarnfromrnJanuaryrntornAprilrn2017;rnStudy participants were required Urine , CSF, wound, Nasal fluid ,Ear ,Blood, Body fluid,rnSemen were collected and cultured on Blood agar, MacConkey agar and chocolate agar andrnincubated according to standard conditions. Then Species identification and antimicrobialrnsusceptibility testing of gram negative bacteria were determined by automated Vitek 2 compactrnsystem using GN and AST GN72 card.rnResult: From the total of 824 various specimens, 284(34.5%) were culture positive , of which (90.8%,rn258/284) of the isolates were fermentative and (9.2%), 26/284) were non fermentative Gram-rnnegative bacteria from wide range of clinical specimens such as in urine 197(69.4%), in wound andrnabscess 34(11.9%), in Ear 20(7.0%), in CSF 11(3.87%), in Nasal 11(3.9%), in Blood 4 (1.4%),rnin Bodyfluid4 (1.4%) and in semen 3(1.1%) .The most common Gram- negative bacterial isolatesrnbeing Escherichia coli 152(53.5%), followed by Klebsiella pneumonia 40(14.1%), Pseudomonasrnaeruginosa 13(4.6%), Proteus mirabilis 13(4.6%), Acinetobacter baumannii 8(2.8%) were thernmost dominant isolate respectively. Among gram negative bacteria, K.pneumoniae (82.5%),rnE.coli (82.9%), P.aeruginosa (100%), and A. baumanii (100%) were multi drug resistance.rnConcussion: High antimicrobial resistance and multi drug resistance was demonstrated over thernstudy period, which may be due to associated with the improper antibiotic consumption or lackrnof a proper guideline for empirical therapy.rnKeywords: Antibiogram, Enterobacteriaceae, fermentative and non-fermenting gram-negativernbacilli, antimicrobial susceptibility test and antimicrobial resistant

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