Hydroponic Screening Of Aluminium Tolerance And Genetic Diversity Analysis Of Ethiopian Finger Millet (eleusine Coracana (l.) Gaertn) Genotypes As Revealed By Inter Simple Sequence Repeat (issr) Markers

Microbial, Cellular And Molecular Biology Project Topics

Get the Complete Project Materials Now! »

Eleusine coracana commonly called finger millet, is an annual allotetraploid (2n=4x=36), thatrnbelongs to grass family Poaceae, subfamily Chloridoideae. Biotic and abiotic stress combinedrnwith the use of less productive local cultivars cause low production in Ethiopia. This researchrnwas aimed to investigate acidity tolerance and genetic diversity of finger millet accessionsrnfrom Ethiopia. The study was done on 288 finger millet accessions obtained from EthiopianrnInstitute of Biodiversity and six national varieties from NARs for optimization andrnpreliminary screening, while 80 accessions were used for further characterization andrnmolecular diversity work. The optimal Al toxicity level identified was 112.50 μM. VarietyrnGute was found to be Al tolerant and used as tolerant standard, while variety Necho wasrnfound susceptible and used as susceptible standard. Shoot and root length (SL, RL) as well asrntotal fresh weight (FW) were measured after eight days of germination, and root length wasrnfound to be seriously affected. Screening was carried out on 288 accessions along with thernstandard checks in six batches, 75 (26.04 %) of them were Al tolerant, while 213 (73.95 %)rnwere from medium to susceptible. After characterization 63 (78.75 %) out of 80 accessionsrnshowed significant Al induced stress in root length, while no distinct and visible symptom ofrnaluminum toxicity were observed in the shoot of finger millet genotypes. According to RTI 21rngenotypes were classified as tolerant, 35 genotypes as susceptible, and 24 as intermediate.rnAnalysis was also carried out to estimate the genetic diversity among and within accessions ofrnfinger millet using six ISSR primers. The total genetic diversity (H) and Shannon’s diversityrninformation index (I) for the entire populations showed, 0.28, and 0.41, respectively.rnIndividuals from Wellega, Gojam, Awi, and Zimbabwe showed the highest level of generndiversity similarly (H = 0.19, I = 0.28), while the lowest variability was shown by accessionsrnfrom Omo (H = 0.05, I = 0.07). Variation within population was higher (58.54%) as comparedrnto that of inter population (41.45%) based on AMOVA. Most individuals from all populationsrntended to form their own cluster, while only few of the individuals were distributed all overrnthe tree. In PCO most of the individual accessions were clustered to their respectivernpopulation. The result of the present study confirmed the presence of acid tolerant andrngenetically diversified accessions that can be used to improve the productivity as well as callsrnfor a combined effort for the collection, conservation and sustainable use of finger millet.rnKeywords: Acidity, Eleusine coracana, genetic diversity, hydroponics, ISSR

Get Full Work

Report copyright infringement or plagiarism

Be the First to Share On Social



1GB data
1GB data

RELATED TOPICS

1GB data
1GB data
Hydroponic Screening Of Aluminium Tolerance And Genetic Diversity Analysis Of Ethiopian Finger Millet (eleusine Coracana (l.) Gaertn) Genotypes As Revealed By Inter Simple Sequence Repeat (issr) Markers

178