Urinary tract infection (UTI) is a common complication of pregnancy. In the majority of thernpatients, infection appears to be confined to thc lower urinary tract. It may be asymptomaticrn(asymptomatic bacteriuria), or symptomatic (acute cystitis). All pregnant women, therefore,rnshould have their urine cultured at t heir first visit tot he clinic. T he aim 0 f this study w as torndetelmine the prevalence of UTI in pregnant women attending antenatal clinic of T ASH, AddisrnAbaba, Ethiopia, and to analyze the antimicrobial susceptibility pattern of bacterial uropathogens.rnA cross-sectional study was conducted on urine specimens collccted from pregnant womenrnattending antenatal clinic of T ASH. Four hundred fourteen pregnant women comprisingrnasymptomatic pregnant women (n=369) and symptomatic prcgnant women (n=4S) wererninvestigated for urinary tract infection from January to March 200S. The age ranges of bothrngroups were 18-44 years, with mean age of 27.1 and 2S.1 years in asymptomatic group and inrnsymptomatic group respectively. Bacteriological screening of mid-stream urine specimenrnrevealed that 3 91}69 (10.6%) and 9/45 (20%) had significant bacteriuria in asymptomatic andrnsymptomatic group, respectively. Thc difference was not statistically significant (p>0.05). Thernoverall prevalence 0 furinary tract infection was 4 8/414 (11.6%). Symptoms did not associaternwith the prevalence of symptomatic urinary tract infection. Prevalence of urinary tract infectionrnwas significantly associated with past history of urinary tract and maternal educational level (prn