Genetic Diversity Oil Content And Fatty Acid Composition Of Vernonia Vernonia Galamensis (cass.) Less Germplasm In Ethiopia

Vernonia (Vernonia galamensis) is a potential novel industrial crop due to high sources of natural epoxidized oil, which can be used for the manufacturing of polyvinyl chloride, petrochemicals, cosmetic and pharmaceutical products. In spite of its high potential as an industrial plant, however, the plant is underutilized. No much attention has been given to research and conservation of this plant. The species is also under the threat of continued genetic erosion. This study was therefore initiated to assess the genetic diversity of V. galamensis accessions through morphological characterization and SSR molecular markers. In addition, oil content and fatty acid composition of the plant were investigated. rnFor agro-morphological trait study, 80 accessions of V. galamensis were grown in alpha-lattice design with two replications at Melka Werer and Wondo Genet Agricultural Research Centers. Data on a total of 13 quantitative traits were collected and analyzed using graph pad prism V-7, SAS V 9.0 and MINITAB V17.1 software. For molecular analysis, 20 SSR markers were used to analyses genetic diversity of a total of 10 populations using GenAlex software. Seed oil content was determined using Soxhlet method, and fatty acid composition was using Gas Chromatography Mass Spectroscopy. Data analysis for seed oil content and fatty acid composition were conducted using SAS V 9.0 and Minitab V17.1 software (Minitab, 2013). rnAgro-morphological quantitative characters have highly significant variation (p

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