Background: Neonatal mortality is the issue of local, regional, national, continental and global.rnFor example, it is observed in both middle- and low-income countries, especially sub-SaharanrnAfrica including Ethiopia. Neonatal death is multi factorial, but the most important single causesrnof neonatal deaths were accounted for preterm birth, birth asphyxia, and sepsis. Effectivernresuscitation, if done timely, can drastically reduce neonatal deaths. rnObjective: To assess the knowledge, attitude and practice toward neonatal resuscitation amongrnmidwives, NICU nurses, Addis Ababa university pediatrics and OBGYN residents in TikurrnAnbesa Specialized, Zewditu Memorial and Gandhi Memorial Hospital, Addis Ababa, Ethiopia. rnMethods: A Cross-Sectional research design was conducted from March â€“ June 2021 and thernrespondents were selected by using simple random sampling techniques, 204 have returned thernquestionnaire filled complete (response rate of 98.07%). The data collection tool was closedrnended questionnaires. Data was verified, coded, and entered to Epi Data Software version 3.1rnand was exported into SPSS version 25 Software for analysis. The degree of association betweenrnvariables was assessed using logistic regression. Finally, p-value less than 0.05 were consideredrnstatistically significant. rnResults: The good knowledge level among midwifes, nurses, pediatrics residents and OBGYNrnresidents were 30.9%, 28.2%, 42.4% and 42.9% respectively. Neonatal resuscitation training andrnthe time training was given, was found to be the only predictor of knowledge score ofrnrespondents (AOR = 3.1, 95 % CI: 1.2 - 4.8, P value = 0.003) and (AOR =5.6, 95 % CI: 1.19 -rn6.72, P value = 0.044) respectively. The majority 58.80% of the participants had good attituderntowards neonatal resuscitation. Whereas the good practice score among midwifes, nurses,rnpediatrics residents and OBGYN residents were 59.8%, 33.3%, 59.6% and 39.4% respectively. rnThe only determining factor associated with poor practice score was lack of availability ofrnresuscitation guideline (AOR=2.55, 95% CI: 1.90-8.28, p=0.004). Having good knowledge werernfound to be the only predictor to have good attitude level (AOR=0.357, 95% CI: 0.18-0.67,rnp=0.001).rnConclusion and recommendations: Knowledge, attitude and practice of nurses and midwifesrntoward neonatal resuscitation were inadequate. Training and the time training given were found to be predictor for knowledge while presence of guideline was predictors for practice.rnContinuous and regular training for health professionals should be strengthen.