Background: histopathology is a gold standard method in pathology where tissue samples are grossed,rnprocessed, cut, stained, mounted and microscopically evaluated. Samples of tissue are taken fromrnpatients and then prepared using appropriate staining protocols to detect and diagnose the disease.rnHematoxylin and eosin is the most pronounced technique. Eosin is a synthetic dye derived fromrnfluorescein. Eosin is hazardous to humans and animals. With the increasing awareness of green earth itrnis advisable to use more of eco-friendly and biodegradable material which can be effectively achieved byrnthe use of natural dye from plants and other natural sources. Turmeric available as curcuma longarn(domestic) is believed to have staining property. rnAim: This study aimed to evaluate the efficacy of turmeric solution as cytoplasmic stain in hematoxylinrnand eosin staining procedure for substitution of hazardous eosin. rnMethod: prospective cross-sectional method and convenience sampling technique was used at TikurrnAnbesa Specialized hospital pathology department in August 2020. Forty tissue blocks were used. Fromrneach block three sections were taken at 4Âµm thickness, a total of one hundred twenty sections groupedrninto three and stained turmeric with mordant (H&TM), turmeric without mordant (H&T) andrnconventional eosin (H&E) staining methods. Staining was evaluated by three histopathologistsrnindependently, using standard checklist. Slides were scored using six parameters: Nuclear, cytoplasmic,rnuniformity, clarity, crispness and background of staining. Slides that satisfied at least four of thernaforesaid parameters were considered adequate for diagnosis. Z- test and chi-square test were used torncompare the difference among the three methods, p-value < 0.05 considered significant. rnResult: TM stained slides were adequate for diagnosis was comparable with that of the conventionalrneosin method. There was no statistical significant difference among pathologist as well as the methods p-rnvalue was >0.05(0.123). On average from a total of 120 stained slides, 112(93.3%) stained slides werernadequate for diagnosis and 8 (6.7%) inadequate. but adequacy and substituting capability of HT withrnthat of eosin showed high discrepancy in adequacy. There was statistical significant difference amongrnmethods p- value was