BACKGROUND:rnObstetric fistula although is eliminated in high income countries, it remains prevalent andrndebilitating condition in many parts of the developing world leaving women with the immediaternconsequences, such as leakage of urine or feces or both and has been observed since women first beganrndelivering children despite of the fact that it is completely preventable if high quality basic andrncomprehensive maternal health services are available to all woman during pregnancy and childbirth by arnwell-trained and knowledgeable skilled birth attendants.rnOBJECTIVE: The objective of this study was to assess knowledge and practice on prevention of ObstetricrnFistula among skilled birth attendants in public Health Centers in Addis Ababa.rnMETHODS: Institutional based Quantitative Cross sectional study was undertaken in public health centersrnin Addis Ababa. A multi staged systematic random sampling technique was implemented to select a totalrnof 548 skilled birth attendants. The data was collected by using self-administered questionnaire preparedrnin English language from March–April 2016 after ensuring that all requirement of Ethical consideration isrnfulfilled. The collected data was cleaned, entered and analyzed using SPSS Version 20. Odds ratios withrn95 % confidence interval with binary logistic regression model was used to measure the association ofrndependent and independent variables. Descriptive statistics with frequency and percentages, table’s graphsrnand cross-tabulations was used.rnResults: This study revealed that 67% of skilled birth attendants had good knowledge on prevention ofrnobstetric fistula and in-service training related to obstetric fistula (AOR =1.53, 95% CI 1.04-2.25),rnresource availability (AOR =2.78, 95% CI 1.46-5.28) and service year (AOR =1.90, 95% CI 1.08-3.36)rnwere significantly associated. About 66.2% of skilled birth attendants had good practice. Diploma nursesrnand degree nurses were less likely to have good practice as compare to Diploma midwives. (AOR =0.41,rn95% CI 0.23-0.74; AOR =0.29, 95% CI 0.16-0.56) respectively. Pre-service training (AOR =2.19, 95% CIrn1.48-3.24), in-service training (AOR =1.59, 95% CI 1.05-2.39), resource availability (AOR =2.85, 95%rnCI 1.44-5.65), and knowledge (AOR =2.47, 95% CI 1.65-3.68) were other factors independentlyrnassociated with practice on prevention of obstetric fistula.rnConclusion and recommendation: In conclusion majority of skilled birth attendants were found to haverngood knowledge and good practice towards prevention of Obstetric Fistula. Obstetric Fistula should bernadequately addressed for health students during their pre-service training and training regarding ObstetricrnFistula and related issues should also be given for skilled birth attendants.rnKey word: Obstetric Fistula Prevention, Skilled birth attendants