Introduction:rnHIV still remains a major challenge globally despite decades of, advocacy,rnawareness raising and investing in programs to control the spread of HIV. ThernGlobal Plan towards the elimination of new HIV infections among children byrn2015 and keeping their mothers alive was well underway, with ambitious goals ofrnreducing the number of new HIV infections in children by 90% and HIV-relatedrnmaternal deaths by 50%. There was now unprecedented collaboration andrnpolitical will to accomplish these goals. The national PMTCT program in Ethiopiarnwas launched in 2001 through implementing the first PMTCT guideline that wasrnfocusing on opt-in approach and use of single dose NVP for the mother and thernbaby. Following the launch of the Global e-MTCT plan in 2011, Ethiopia hasrnlaunched the accelerated plan of PMTCT in 2012 focusing on site expansion,rnquality improvement, demand creation and use of more efficient PMTCT regimenrnand adapted PMTCT option B+ .rnObjective: To assess knowledge, attitude about PMTCT option B+ and associatedrnfactors among ANC clients in Dessie.rnMethod: A cross sectional study design that employed simple &systematicrnrandom probability sampling, quantitative data collection method supplementedrnby qualitative FGD conducted on a sample size of 301 study subjects from marchrn14 to April16 2015G.C. Descriptive, bivariate and multivariate logistic regressionrnanalyses were conducted. Statistical tests were done at a level of significance of prn= 0.05. Tables and graphs were used to present result.rnResults: In this study 297 (99.7 %) of the required sample pregnant womenrnattending antenatal care were participate 199(66.1%) had positive attitude andrn167(55%) had the knowledge of HIV/AIDS transmission ways. 168(55.8%) werernknown what the service PMTCT option B+ mean, however, 129(42.9%) were notrnhave clear understanding. 243(80.7 %) study participants thought to receivernreactive results face to face without their husband. Professional women were 4.53rntimes [Adj.OR & (95%CI) = 4.53(1.23-15.31)] more than 1-4th grade womenrnpositive opinion for the PMTCT option B+ services utilization and Muslimrnfollowers were 0.03 times [Adj.OR & (95%CI) = 0.03(0.15-0.83)] more positivernopinion on PMTCT option B+ services utilization than orthodox followers.rnConclusion: Most of the study participants in this study knew that HIV could berntransmitted from an infected mother to her baby and most of them know what didrnthe service PMTCT option B+ mean and its benefit and they had also positivernattitude on PMTCT option B+ service but worried about discrimination by theirrnpartner.rnKey words: Prevention of mother to child transmission option B+ Antenatal care