BACKGROUND: Colostrum helps to prevent bacterial infections that are a danger to newbornrnbabies. The antibodies probably help to prevent a baby from developing allergies. From aroundrnthe world statistics reveal that colostrum is frequently discarded. Lack of knowledge regardingrncolostrum feeding is the major cause for infant morbidity and mortality rates. There are diversernfactors that affect colostrum feeding in Ethiopia; socio-demographic factors, obstetric factors andrnhealth service-related factors, and traditional beliefs. rnOBJECTIVE: To assess colostrum feeding practice and associated factors among postnatalrnmothers in selected health facilities, in Addis Ababa Ethiopia. rnMETHODS: Facility-based cross-sectional quantitative study was conducted since March 27, tornApril 30, 2020.Totally ten health centers selected. The Sample size was calculated by using arnsingle proportion formula and the total 427sampled post-natal mothers were participated in thisrnstudy and allocated to each selected health centers proportion to size. The data was collectedrnusing self-administered questioners. The analysis was done by SPSS version 26 for data entryrnand analysis. Both bivariate and multivariate logistic regression analyses were used to identifyrnfactors associated with colostrum feeding. rnRESULT: The prevalence of colostrum feeding practice was 88.8%, which was higher than therncountry recommendation level. Mothers who had ANC visiting more fed colostrum than mothersrnwho had not ANC visiting (AOR=5.241(95%CI.1.797, 15.286).Mothers who had a goodrnperception of colostrum more fed their babies than those who had poor perception (AOR=7.433rn95% CI3.021, 18.289). rnCONCLUSION AND RECOMMENDATION rnThe prevalence of colostrum feeding practice was 88.8%, grater majority of mothers practicedrncolostrum feeding, which was higher than the country recommendation level rnMaternal perception, sources of information, ANC visiting, associated factors for colostrumrnfeeding practice. Health education programs should be given for on ANC follow-up regardingrnthe importance of colostrum feeding. rnMass media should also take a step to educate the families and mothers about colostrum.rnMinistry of health to take action increasing the promotion of breast-feeding practice especiallyrnbenefits of colostrum feeding.