Evaluation Of Wound Healing Activity Of 80 Methanol Leaf Extract Of Vernonia Leopoldi (sch. Bip. Ex Walp.) Vatke (asteraceae) In Mice

Vernonia leopoldi (Sch. Bip. Ex Walp.) Vatke locally known as “Nechilo” is a shrub that is rnendemic to Ethiopia. Traditionally, it is used for the treatment of wounds. However, there is nornscientific evidence that justifies the traditional claims. Thus, the present study assessed thernwound healing potential of 80% methanol leaf extract of V. leopoldi in mice. rn rnExtraction of the leaf was carried out by maceration using 80% methanol. The extract wasrnstudied for wound healing activity by formulating a topical ointment using two concentrationsrn(5% and 10% w/w). Simple ointment base (negative control) and nitrofurazone 0.2% (w/w)rnointment (positive control) were used for comparison of wound healing activity in mice. Twornwound models were used i.e. excision and incision. For anti-inflammatory study, carrageenaninducedrnhindrnpawrnedemarnmodelrnwasrnused.rnThernextractrnofrnV.rnleopoldirnwasrngivenrnorallyrnatrndosesrnofrnrn100,rnrn200 and 400 mg/kg. The anti-bacterial activity of the extract was determined using diskrndiffusion technique against both gram positive and gram negative bacteria that are associatedrnwith wound at a concentration of 50-500 µg/µl. Treatment of wound with ointment containingrn5% and 10% (w/w) extract exhibited significantly increased (p

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