Wound Healing And Anti-inflammatory Activities Of Solvent Fractions Of 80 Methanol Leaf Extract Of Achyranthes Aspera L.(amaranthacea) In Rats

The leaf of Achyranthes aspera L.(Telenj), has been used traditionally for the treatment of wound in var-rnious parts of Ethiopia. The leaf had many confirmed in-vitro and in-vivo activities that can promotewound healing and anti-inflammatory effects. However, the various fractions of A. aspera have not beenrnexplored scientifically for its wound healing and anti-inflammatory activities in animal model. The objectivernofrnthisrnrnstudy was to evaluate the wound healing and anti-inflammatory activities of solvent fractionsrnrnof 80% methanol leaf extract of A. aspera in rats. Air dried leaf of A. aspera were grounded andmacerated for 72 hours successively by 80 % methanol three times. Then the dried extract was fractionatedrnwithrnrnchloroform, n-butanol and distilled water. Acute oral and dermal toxicity was determined inrnrats. Wound healing activity of solvent fractions was evaluated using excision and incision wound models.rnrnAnti-inflammatory activity was evaluated using carrageenan induced hind paw edema and cottonpelletrnrninduced granuloma models in rats. 2000 mg/kg oral and 10 % w/w solvent fraction ointment testdoses were safe in rats. The 10 % w/w chloroform fraction ointment revealed high percentage of woundrncontraction (p

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