Ciprofloxacin is clinically important fluoroquinolone, effective against Escherichia coli (E. rncoli) infections across the globe. However, many clinical isolates of E. coli have emerged asrnresistant to ciprofloxacin, restricting therapeutic options. Due to the paucity of newrnantimicrobial agents in the drug development pipeline, it is imperative to develop newrnalternative approaches that improve antibacterial efficacy of the available antibiotics. The aimrnof the current study was therefore to biosynthesize silver nanoparticles (AgNPs) usingrnaqueous extract of Aloe camperi for ciprofloxacin delivery, thereby enhancing its efficacyrnagainst ciprofloxacin resistant E.coli. In this study, the aqueous extract of Aloe camperi wasrnutilized as a reducing and capping agent for the synthesis of AgNPs. Crucial operationalrnparameters were controlled. Ciprofloxacin was loaded on the surface of AgNPs and thernencapsulation efficiency was determined. Free and ciprofloxacin loaded particles wererncharacterized by VU-visible spectroscopy, fourier-transform infrared, dynamic lightrnscattering, scanning electron microscope, X-ray diffraction and simultaneous differentialrnscanning calorimeter-thermogravimetric analysis. The in vitro release profile of ciprofloxacinrnfrom the surface of AgNPs was investigated. Furthermore, the in vitro susceptibility test ofrnthe loaded particles as compared to its individual components was evaluated by employingrndisk diffusion test. The results of characterizations revealed a successful synthesis ofrncrystalline AgNPs with an average hydrodynamic diameter of 98.9 nm Â± 0.3. Ciprofloxacinrnwas also effectively loaded on the surface of AgNPs with a maximum encapsulationrnefficiency of 60.94 %. The in vitro releasing profile of ciprofloxacin exhibited a biphasicrnpattern at all study pH conditions. However, the releasing rate was pH dependent. Afterrnloading, the susceptibility of E.coli against ciprofloxacin was transformed from resistant tornintermediate. Therefore, this study demonstrated that biosynthesized AgNPs using Aloerncamperi aqueous extract could be a potential nano carrier for ciprofloxacin delivery tornenhance its efficacy against ciprofloxacin resistant E.coli.