Hptlc-densitometric Method Development And Validation For Simultaneous Determination Of Abacavir Lamivudine And Zidovudine In Combined Dosage Form

A simple, accurate and precise HPTLC-densitometry method for thernsimultaneous determination of abacavir, lamivudine and zidovudine inrnpharmaceutical tablets has been developed. Separation was performed onrnaluminum backed HPTLC plates (silica gel 60 F254 with 0.2 mm thickness,rnCamag, Muttenz, Switzerland) using methanol: chloroform: acetonitrile (4 : 8: 3rnv Iv) as a solvent system. Rf values were observed to be 0.38, 0.58 and 0.71 for 3-rnTC, ABC and AZT respectively. Good sensitivity for all analytes was observedrnwith UV detection at 275 nm and the solvent front and saturation time were 80rnmm and 25 minute, respectively. The method was validated for linearity,rnaccuracy, precision, peak purity test and robustness. The method allowedrnquantitation over the 200-1450 ng/band range for all abacavir, lamivudine andrnzidovudine. Both linear and second order polynomial relationship were studiedrnand second order polynomial fit was found to be more suitable, its residuals plotrnshowed a much better fitting than that of linear model, indicating goodrncorrelation and determinations (r = 0.99995, 0.9998 and 0.9998 and r2 = 0.9999,rn0.9997 and 0.9996 for lamivudine, abacavir and zidovudine, respectively). Thernmethod has an accuracy of 99.35, 99.19 and 99.13 % for lamivudine, abacavir andrnzidovudine, respectively. The method is robust and has the potential torndetermine these drug substances simultaneously from the dosage forms with outrnany interference.

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