Anticonvulsant Effect Of Pterlobium Stellatum (leaves) Moringa Stenopetala (root) And Clutia Abyssinica (leaves) Traditionally Used For Treatment Of Epilepsy In Ethiopia Using Mice Model

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Background :Epilepsy is the third leading contributor to the global burden of disease for rnneurological disorders and affects 65 million people worldwide. Although the current rnantiepileptic drugs achieve symptomatic seizure relief they do not prevent or reverse the rnpathological process that underlies human epilepsy. Drug resistant epilepsy is also one of the rnmost important unmet needs in the daily management of epilepsy. These currently unmet needs rnprovide a roadmap for the development of more effective antiseizure drugs, as well as for disease rnmodifying and antiepileptogenic drugs especially from plants. This study was conducted to rninvestigate the anticonvulsant activity of the crude hydroalcoholic extracts of 3selected plants rnPterlobium stellatum ( leaves), Moringa stenopetala (root) and Clutia abyssinica (leaves). These rnall are used for treatment of epilepsy by traditional healers in different parts of Ethiopia. rnAdditionally the solvent fractions of the Pterlobium stellatum were tested for anticonvulsant rnactivity as the crude extract showed positive response in all the models used. rnMethods :.The dry residues of the plant extracts were used for test in different doses. Male balb rnc mice were used for in vivo study and for in vitro study P14-P21 of C57BL16 mice were used. rnIn vitro mice model of hippocampal slice with 0Mgrn2+rn was used and the extracts were tested at rnthe 0.7mg/kg concentration. Diazepam 3µM was positive control and DMSO as negative control. rnIn in vivo PTZ and MES mice models 400mg/kg and 800mg/kg of each test extract were used for rnefficacy test in as positive control Diazepam 5mg/kg and phenytoin 10mg/kg were used rnrespectively. The negative control was 2% tween 80 .Fisher's exact test was used to analyze rnproportions and ANOVA with post hoc LSD to test means. The tests were conducted after the rnethical clearance was obtained from the ethical committee of Addis Ababa University and rnuniversity of Cape Town. Qualitative test and quantitative tests were used to determine the secondary metabolites in the plants. And ultraperformance liquid chromatography-mass rnspectrometer (UPLCMS) tests were used to characterize plant constituents in Pterlobium rnstellatum crude extract and its fractions. rn rnResults :In the in vitro study the hydroalcoholic extract of P.stellatumand M. stenopetalaat rn0.7mg/ml had a statistically significant anticonvulsant activity compared to negative rncontrol(P

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Anticonvulsant  Effect Of  Pterlobium Stellatum (leaves) Moringa Stenopetala (root) And Clutia Abyssinica (leaves)  Traditionally Used For  Treatment Of Epilepsy  In Ethiopia Using  Mice Model

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