The four objectives of this PhD study were to develop protocols for embryo rescue technique, efficientrnplant regeneration system from immature embryos, in vitro haplodization either through androgenesisrnor gynogenesis and transformation in Eragrostis tef. The major study materials include were three tefrnvarieties, improved, DZ-01-196 and DZ-CR-37, a farmers variety, Fesho and the wild, relatives Ernmexicana, .E, pilosa and E.papposa. For embryo rescue study, both zygotic embryos and ovaries atrnearly stage of self pollination were excised and cultured in the medium. More than 90 % rescue wasrnrecorded for tef varieties from both explants. Similar results were obtained from some wild speciesrnfrom embryo culture. DZ-01-196 performed better than DZ -CR-37 and Fesho in both explant types.Arnhighly efficient in vitro culture system was also developed using immature embryos of tef. Efficiencyrnwas examined by the embryogenic calli formation, plant regeneration and number of shoots perrnexplant obtained. 100 % somatic embryo formation, 98.2% plant regeneration as well as maximumrnnumber 565 plantlets per single explant were obtained from variety DZ-01-196. This highly efficientrntechnique developed here will be very useful for further studies like transformation which requiredrnhigh plant regeneration system. In androgenesis study factors influencing androgenic response wererninvestigated. 0.15 % of calli were induced from the cultured microspores and only one albino plantrnwas obtained. This result showed that tef is recalcitrant to androgenesis. Therefore, in vitrorngynogenesis was attempted. Out of 14234 cultured unpollinated pistils, 2035 embryonic tissues andrn13.4 %. regeneration were obtained The flow cytometry analysis of these plants revealed 5 haploidsrn(di-haploids), 2, aneuploids, 172, tetraploids and 1 octoploid plant. Haploid and variant polyploidyrnplant production in tef is for the first time. For tef transformation study, genes for selectable markerrnand reporter genes were used and 4 transformed plants from biolistic and 1 transgenic plant fromrnagrobacterium method survived the repeated sub culturing on selection media. Transgenic plants,rnrecovered after 4-6 weeks from the time of gene transfer were subjected to PCR and southern blotrnanalysis. The result revealed from T1 plants stable transformation from both transformation system.rnThis protocol will contribute for the improvement of the crop in the near future.rnKey words: Eragrostis tef, ovary, immature embryo, rescue, pistil, microspores, haplodization,rnandrogenesis, embryonic tissue, gynogenesis, biolistic and agro-bacterium transformation.