The crude chloroform, ethyl acetate, acetone, methanol and ethanol extract of the leavesrnof Cucumis ficifolius and Zehneria scabra were tested against in vitro antibacterialrnactivity of five human pathogenic bacteria using disc diffusion method and agar dilutionrnmethod for Minimum Inhibitory Concentration (MIC). Pseudomonas aeruginosa andrnShigella boydii were susceptible test organism for the ethyl acetate and acetone extractsrnof the leaves of C. ficifolius with inhibition zones of 14Â±0.577mm, 12Â±0.577mm and anrnMIC of 0.781mg/ml, 3.125mg/ml respectively, but Escherichia coli and Salmonella typhirnwere the most resistant bacteria to all extracts of the leaves of this plant species with norninhibition zones. Staphylococcus aureus and E. coli were found to be the mostrnsusceptible bacteria for the ethyl acetate and acetone extracts of the leaves of Z. scabrarnwith inhibition zones of 22.6Â±0.33mm, 14Â±0.577mm and MIC of 0.781mg/ml,rn1.56mg/ml respectively. However S. typhi was the most resistant to all extracts of thernleaves of this plant with no inhibition zone. The acetone extracts of C. ficifolius and thernethyl acetate extracts of Z. scabra were most potent but the chloroform extracts of bothrnmedicinal plants were ineffective against all bacterial and fungal test organisms. C.rnficifolius and Z. scabra didnâ€™t show any inhibitory activity against Botrytis and Fusarium.rnIt can be concluded that C. ficifolius and Z. scabra had shown antibacterial activityrnhaving potential for further study to serve as source of antibacterial agents.rnKey words and phrases: Antibacterial activity, antifungal activity, minimum inhibitoryrnconcentration, Cucumis ficifolius, Zehneria scabra.