A study on the extent and pattem of genetic variability among long maturing sorghum genotypesrncollected from Wello and Hararge areas of Ethiopia was conducted using morphological andrnrandom amplified polymorphic DNA (RAPD) markers. The field experiment was conducted at thernexperimental field of Melkassa Agricultural Research Center and the molecular work was done atrnAnnauer Hanson Research Institute laboratory, AddisAbaba. A total of 27 morphological traitsrnwere recorded from all 26 genotypes included in the study. RAPD marker analysis was conductedrnon fourteen genotypes sub-sampled, from the different clusters generated using morphologicalrndata. Distribution of various qualitative traits between regions and altitudes zones showed variationrnfrom total absence to high level of variation from one morphological trait to the other. MeanrnShannon-Weaver diversity index (H) varied from 0.04 for grain plumpness to 0.99 for seed colorrnwith over all mean of 0.60. Highest heritability and genetic advance was observed for leaf area,rnplant height, number of primmy branches and number of seeds per head, indicating that selectionrnprogress will be expected to be high for these traits. High heritability coupled with high expectedrngenetic gain may be due to high additive gene effects implying that selection applied for theserntraits would lead to yield in an improvement in . the crop of study. Variation within accessions,rnregions of origin and altitude classes accounted for a large portion of the total variation forrnmorphological data. But, Nie's diversity index from molecular marker data fails to reflect thernwithin accessions and region variation, rather it showed relatively higher variability betweenrnpopulations and regions of origin. Cluster analysis from morphological data fail to grouprngenotypes either based on their region of origin or altitude classes while cluster from molecularrnmarker tend to arrange genotypes based on their regions of origin regardless of their altitudernclasses.