This study was aimed at the development of antimicrobial packaging films, which is one of thernmost promising active packaging systems, from aloe and papaya leaves extracts with gelatinernand glycerol. Three different extracts of papaya leaves were analyzed for their antimicrobialrnactivities. Mechanically obtained papaya leaf extract showed the best result (12.10mm inhibitionrnzone on S.typhi). Based on antimicrobial activity, film forming ability, transparency, and colour;rnAloe debrana extract was found to be more appropriate for the development of antimicrobialrnpackaging films than Aloe trichosantha extract. The antimicrobial activity of Aloe debranarnextract was increased by the incorporation of papaya leaf extract up to 30%, above which it didrnnot bring significant influence on most test organisms. As a result 70% Aloe debrana extract andrn30% papaya leaf extract standard solution was prepared. Various concentrations of gelatine andrnglycerol were added to the standard solution for packaging films development. Films werernevaluated for their antimicrobial activities, physicochemical, and mechanical properties. Thernantimicrobial activities of aloe based films were tested on E. coli, S. typhi, S.aureus, C. albicans,rnand F. xylarioides. All films exhibited inhibitory zones on the test microorganisms used in thisrnstudy. A wide inhibition zone (6.52cm2) was observed on S. typhi growth whereas the least (4.20rncm2) was on C.albicans. Films were soluble in water with highest solubility (90.49%) for P1,1rn(film with 1g glycerol and 1g gelatine) and lowest (44.57) for P0,2(film developed by adding onlyrngelatine). Film solubility significantly increased as the concentration of glycerol increased andrndecreased as the concentration of gelatine increased. Film P0.5,2(with 0.5g glycerol and 2grngelatine) has showed maximum tensile strength (65MPa) where as the lowest (20MPa) wasrnobtained by film P0.5,1(0.5g glycerol and 1g gelatine). Increasing gelatine concentrationsrnsignificantly increases the tensile strength but glycerol has an opposite effect on the tensilernstrength. All the films were highly flexible and stretchable. Film P1,2(1g glycerol and 2g gelatine)rnshowed the maximum elongation (180%) and the minimum elongation (89%) was obtained forrnP0.5,1. This study demonstrated the technological feasibility of developing antimicrobialrnpackaging films from aloe and papaya leaves extracts.rnKeywords: Aloe extracts, Antimicrobial packaging film, Mechanical properties, Papayarnextracts, Pathogenic microorganisms, Physicochemical properties