Worldâ€™s energy needs are largely met by use of non-renewable resources such as petrochemicals,rnnatural gas and coal. Increasing energy demands, environmental concerns and depletion of fossilrnfuel reserves has led to the search for alternate fuels.rnThis work was done with the aim of producing biodiesel from pumpkin seed oil by usingrnmethanol with alkali catalyst, sodium hydroxide. The effects of catalyst amount from 0.22g torn0.99g, molar ratio of methanol to oil from 6:1 to 12:1 and reaction temperature from 30 torn75 on biodiesel yield was investigated. Pumpkin seed oil was extracted using solventrnextraction (using hexane as a solvent). rnThe extracted oil was purified through degumming, neutralization, washing and dryingrnsequentially. Acid value, amount of free fatty acid, saponification value, kinematic viscosity,rnhigher heating value, iodine value and flash point of the extracted oil were determined. Biodieselrnwas produced from pumpkin seed oil using anhydrous methanol 99% (w/w) and sodiumrnhydroxide catalyst 98.6% (w/w). rnThe experimental design was done by using the Design Expert 7.0.0 software three levels; threernfactor Central Composite Design with full type in the optimization study, requiring 20rnexperiments. To determine the effect of temperature, amount of catalyst and molar ratio ofrnalcohol to oil experiments were done in the ranges of 30 to 60 , 0.22g to 0.99g and 6:1 to 12:1rnsubsequently. The maximum biodiesel yield was 88.7 % (w/w) at 45 , 9:1 molar ratio ofrnalcohol to oil and 0.99g amount of sodium hydroxide catalyst. In contrast, the minimumrnbiodiesel yield was 38% (w/w) at 60 , 12:1 molar ratio of alcohol to oil and 1.76g mass ofrncatalyst. The viscosity, density, flash point, acid value, saponification value, higher heatingrnvalue, iodine value Cetane number, moisture content and ash content of the produced biodieselrnwere determined. These properties were matched with ASTM and EN standards specifications