The release of brewery wastewater sludge with high pollutants and soluble organic contents canrnpose a significant threat to human health and environment due to their toxicity. The present studyrnaims at investigating the potential of Microbial Fuel Cell (MFC) to utilize brewery wastewaterrnsludge as feed stock of electricity generation. The characterization of the brewery wastewaterrnsludge before and after it goes through the microbial fuel cell was analyzed using reactor digestionrnmethod, OxiTop BOD5, (HACH method DR890) molybdovanadate method, (HACH method DRrn890) TNT per-sulfate digestion method for chemical oxygen demand (COD), biological oxygenrndemand (BOD), total nitrogen (TN), and total phosphorus (TP) respectively. The effect of varyingrnsalt concentrations (1 M, 2M, and 3M) of the salt bridge in MFC have been analyzed with differentrnfactors like temperature ranging 20 to 45 Â°C, and pH ranging from 4 to 10. Results were analyzedrnin terms of efficiency in chemical oxygen demand (COD), biological oxygen demand (BOD5),rntotal nitrogen (TN), and total phosphorus (TP) removal and capability of energy generation. Thernoptimum temperature was found 32.5 Â°C, with the optimum pH of 7 and 3 M salt bridgernconcentration in which the maximum removal percentage and power generation was observed. Atrnthe optimum condition the observed power output, removal percentage of chemical oxygenrndemand (COD), biological oxygen demand (BOD5), total nitrogen (TN), and total phosphorus (TP)rnwere 0.88 V, 93.58%, 93.07%, 1.39%, and 1.19% respectively. Box-Behnken experimental designrn(BBD) has been used to find the optimum condition for voltage production, chemical oxygenrndemand (COD), biological oxygen demand (BOD5), total nitrogen (TN), and total phosphorus (TP)rnremoval percentage.