Background: Musculoskeletal disorders are one of the serious health problems that frequently occur inrnworkplaces. Female workers are engaged more dominantly in garment factories and highly affected inrnmusculoskeletal disorder. The problem was frequently reported in the growing textile industries ofrndeveloping countries especially among the garment workers. The magnitude and associated factor ofrnwork-related musculoskeletal disorder of female garment workers who worked in a prolonged sittingrnand standing work posture was not adequately investigated in Ethiopia.rnObjective: The study aimed to assess the prevalence and associated factors with work-relatedrnmusculoskeletal disorder among females working in sitting and standing work posture of the garmentrnGalan city, Oromia, Ethiopia.rnMethods: An institutional-based cross-sectional study was conducted from May 1, 2021 to 30, 2021rnamong 410 female garment workers selected from factories located at Galan city, Oromia, Ethiopia.rnFour data collectors and one supervisor were involved in data collection. A pre-tested standardizedrnNordic Musculoskeletal questionnaire was implemented by the interviewer-administered for datarncollection. A simple random sampling method was used to recruit study participants from the selectedrngarments. Data was entered into EPI info version 7, analyzed, and cleaned by using SPSS version 23.rnFrequency tables, graphs, and descriptive summaries were produced to describe the study variables. Arnmodel fitness, collinearity test, and multiple logistic regression of variables were done. Independentrnvariables with a p-value less than 0.05 under 95% CI were considered as having a significant associationrnwith the dependent variable.rnResult: The overall prevalence of work-related musculoskeletal disorders during the last one year wasrn64% with 95% C.I: (59, 68). Lower back is the most commonly affected body region (51%), followedrnby neck (43%) and shoulder (38%). Factors such as always repetitive work [AOR: 2.6, 95% CI (1.1-rn5.2)] , working hour per week [AOR: 2.9, 95% CI (1.8-3.4)] ,Always high loaded work [AOR: 2.4, 95%rnCI (1.1-4.0)], sitting work posture [AOR: 1.8, 95% CI (1.1-3.0)] , ages above 29 years[AOR: 2.8, 95%rnCI (1.1-7.9)] , service above 10 years [AOR: 3.0, 95% CI (1.4-4.8)], other than garment work of doingrnhome activities [AOR: 2.8, 95% CI (1.4-4.6)] , alcohol drinking behavior [AOR: 3.5, 95% CI (1.2-9.6)],rn,job stress[AOR: 2.8, 95% CI (1.3-4.5)] and job satisfaction [AOR: 2.9, 95% CI (1.3-6.3)] werernsignificantly associated with musculoskeletal disorder.Conclusion: A higher prevalence of work-related musculoskeletal disorder is reported, about two-thirdrnof female garment workers had been suffering. Personal and work-related factors are significantlyrnassociated with work-related musculoskeletal disorders. Therefore, it should be use work rotation, torndecrease repetitive and high loaded work exposure, give attention to health and safety rules ofrnergonomics issues.