Background – Mother to child transmission remains the most significant route of HIV infectionrnamong children. For the prevention of mother to child transmission of HIV a package of servicesrnincluding HIV counseling and testing, provision of antiretroviral prophylaxis drugs for mothersrnand babies, safe delivery practices and infant feeding counseling is made available. Therneffectiveness of PMTCT program largely depends on utilization of prophylactic antiretroviralrndrugs by the women and their babies.rnObjective: This study was intended in general to assess the uptake of antiretroviral prophylaxisrnamong HIV positive parturient women (and specifically to determine the magnitude ofrnantiretroviral prophylactic uptake, to describe factors affecting the uptake and to describe thernextent of partner involvement) in South Wollo Zone, East Amahra Region, Northeast Ethiopiarn.Methods: Institution-based cross sectional study was conducted among 356 HIV positivernpregnant women at five selected health facilities in South Wollo Zone,East Amhara Region,rnNorth east Ethiopia between January 2010 and March 2011. All clients were interviewed untilrndecided sample size obtained. Descriptive statistics was used to assess uptake of antiretroviralrnprophylaxis. Associated factors with uptake were assessed using odds ratios and 95%rnConfidence Intervals. Four in-depth interviews/among PMTCT services counselors and WoredarnHealth Office PMTCT coordinators/ were conducted to complement the quantitative survey.rnResult - Of 356 HIV-positive pregnant women interviewed, 151(42.4%) started antiretroviralrnprophylaxis, 101(28.4%) were initiated on combination ART and clinical care beyond PMTCT.rnThirty two (8.9%) their gestational age was less than 28 weeks. 72(20.2%) did not startrnantiretroviral prophylaxis at their 28 weeks of gestational age.rnConclusion- -Accessibility of comprehensive PMTCT services have paramount benefits in effectivernimplementation of ARV prophylaxis uptake. Much of significant associations between variables did notrnshow. It might be related to limitation of study design used. Stigma was not considered as a barrier forrnARV prophylaxis in this particular study area.rnKey words – HIV-positive pregnant woman, PMTCT, ARV prophylaxis.