This study was conducted to develop and evaluate serological tests for C. bovis for therndiagnosis in live animals and determine the prevalence in Addis Ababa Abattoir. Postmortemrninspection (PMI), indirect hemagglutinatio test (IHAT), indirect Enzyme-LinkedrnImmunosorbent Assay (ELISA) and fecal examination techniques were conducted. A total ofrn743 serum samples, 522 from Addis Ababa Abattoir, 101 from Repi PLC Dairy Farm and 109rnfrom Yeka-abado Farmers Association were collected and 11 negative controls from France.rnPostmortem inspection was conducted on cattle slaughtered from Addis Ababa abattoir, 39rn(7.4%) were positive for C. bovis. The hearts of these animals were thoroughly inspected andrnlive C. bovis cysts were collected for antigen preparation. The protein concentration of therncyst was measured using the Lowry method. Indirect ELISA was conducted on knownrnpositive and known negative samples and due to shortage and lack of fresh reagents ELISArnwas not conducted on the other samples. The cut-off was 0.84 with 100% and 81% sensitivityrnand specificity respectively. Parallely IHAT was conducted on positive and negative samples,rnand a titre of 1:64 and above was considered as positive. IHAT had 100% and above 91%rnsensitivity and specificity respectively and when compared with ELISA it showed betterrnspecificity. Based on this test 149 (28.5%) from the Abattoir, 33 (30.2%) from Yeka-abadornFarmers Association and 8 (7.9%) from Repi PLC Dairy Farm samples were positive for C.rnbovis. The prevalence in the two management systems, namely Yeka-abado FarmersrnAssociation and Repi PLC Dairy Farm, was significantly different (p