Bovine brucellosis is an infectious disease of economic importance by causing abortions andrninfertility. Bovint: brucellos is considered a zoonosis. Infprmation on the extent of bovinernbrucellosis. however. is scarce for the expanding intra- and peri- urban dairy enterprise inrnEthiopia. The purpose of this study was to investigate the status of bovine brucellosis and itsrnimpact on reproduction in some intra- and peri-urban dairy productionrnsystemsrnin Ethiopia. Arncross-sectional investigation of bovine brucellosis was carried out from Jun to October 1917rnon -42 dairy farms located in Debre Zeit, Kalili, Sebela, and Addis Ababa. The farms representrnperi-urban systems. Intra-urban systems in secondary towns and intra-urban productionrnsystems. Bulk milk samples were collected from each farm (herd ) in June, August, andrnOctober Blood samples from 950 non-vaccinated breeding animals above six months of agernwere collected once in June. The milk Ring Test (WRT), the Rose Bengal Plate Test (ROPT).rnand the Complement Fixation Test (CFT) were carried out on milk and serum samples. Farmrnmanagement parameters and zoonotic aspects were cullected by administering arnquestionnairern.rnAn overall herd infection rate of 33.3% was determined. With respect to the productionrnsystems 100%. 30%, and 12.5% of the farms were infected in peri-u rban. intra.-urbunrnstecondary town. and intra -urban systems, respectively. The within herd prevalence rangedrnfrom 0-16.7%. At least one or two reactors were idemilied in 64.3% of the farrms. An overallrnprevalence rate of 811% was found on individual animals. A prevalence rate of 9.8 % wasrnobserved in the age group 2 to 4 years. A prevalence rale af 9.1 % and 3.3% was identified inrnfams that keep more than 100 animals, and less than 50 animals, respectively. Significantrndifference in prevalence rate was observed in animals aged below and above 2 yeas andrnbetween no parturition and at least one parturition. A significant difference in prevalencern raternwas also observed between farms that keep below 50 and above 100 animals. Odds ratiosrn(OR) of 2.1 4 (1.04Â·483) between age and brucellosis infection and 2.99 ( 1.29Â· 8.06) betweenrnherd size and brucellosis infection were determined. Significant differences in prevalencernrates were not observed among the production systems and districts in indvidual animals.rnAgreement (kappa values) between RBPT +/'CFT+ and MRT tests were 0.31 (first MRTrnresult ), 0.43 (first and second MRT results pooled), and 0.58 (first. second and third MRTrnresults pooled). Moderate agreement (k=0.44) was found between RBPT+ and CFT +andrngood agreement (k=O.74) was found between RBPT+ and RBPT +ICFT + testing procedurernConsidering positive results in both RBPT and CFT as gold standnrd the sensitivity (SE) andrnspecificity (SP) of the pooled MRT were 57.1% and 89.3%respectively). Sensitivty andrnspecilicity, of RBPT were 100%rnand 94.6%. respectively.rnAssociations betweer reproductive and production parameters and brucellosis infection could rnnot be established for individual animals. However. on the basis of questionnaire .answers,rnprevious abortions and use of natural service were associated with Brucellar infection: OR ofrn15.58 (1.72-79.16) and 0.06(0Â·0.61) were calculated respectivelyÂ·. Abortions were recordedrnin 56.1 % of the farms. Lack of knowledge of brucellosis was recorded in 87.8% of the farms.rnCleaning and disinfection was done regularly in 29.3,% removal of infected animals was farmrnpractice in 14.6%provision of maternity pens was found in 4,9% of the farms, andrn 21.9% ofrnthe farms obtained repbcement stock from outside source.rnProkmged fever was observed in nine persons interviewed . They were attending cows for atrnleast six months.rnAbsence of vaccination . herd size, cuttle density, lack of knowledge of brucellosis, and poorrnmanogement and husbandry practices are likely factors responsibl to explain the relaivelyrnhigh prevalence rate observed in his study. Due to its impact on production and lfor itsrn'Zoonotic significance. Awareness of brucellosis in urban farming should be given priority.rnConclusions and recommendations in this regard, emphasizing control of brucellosis arerngiven.