Isolation Molecular Characterization And Sero-prevalence Study Of Foot-and-mouth Disease Virus Circulating In Central Ethiopia

A cross sectional study was conducted to determine sero-prevalence and to isolate and characterize causative agents of foot-and-mouth disease (FMD) in selected areas of Central Ethiopia from September 2015 to May 2016. In addition, the study was meant to assess epidemiological risk factors associated with FMD in dairy cattle. A multistage random sampling for the sero-prevalence study was implemented to select the required sample size. Blood sample was collected in vacutainer tubes for sera separation and the sera were tested by 3ABC-Ab ELISA. Outbreak cases were investigated by collecting epithelial tissues and by using oro-pharyngeal fluids samples for FMD virus isolation and characterization. The isolated viruses were used for antigen typing by sandwich ELISA. Molecular characterization was done by VP1 gene sequence generation and phylogenetic tree reconstruction. Laboratory results were analyzed using descriptive statistics, chi-square test and logistic regression. The overall sero-prevalence of FMD was 24.22% (139/574) for the dairy cattle. Sero-prevalence of 26.84% and 22.91% was recorded in Adama and Asella, respectively but not significantly different (P>0.05). In logistic regression analysis, breed, age and animal composition were significantly associated with FMD sero-prevalence (p

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