Evaluation Of Potency And Immunogenecity Of Inactivated Cell Culture Rabies Vaccine Prepared At National Veterinary Institute Ethiopia

Rabies is the first prioritized zoonotic disease threatening public health in Ethiopia. Massrnvaccination is the best existing strategy to control canine rabies. The objective of thernpresent research was to evaluate potency and immunogenicity of Evlelyn-Rokitnicki-rnAbelseth (ERA) inactivated cell culture anti-rabies vaccine producing in NationalrnVeterinary Institute (NVI). The vaccine was produced as per the institute’s productionrnstandard operation procedure (SOP). Potency test of the vaccine conducted in SwissrnAlbino mice according to National Institute of Health, America (NIH) potency testrnprocedure and Verorab rabies vaccine was used as reference vaccine. However, thernimmunological experiment undertaken in three to four months old puppies. Randomlyrnassigned eight puppies to the vaccinated group were subcutaneously administered 1ml ofrnNVI’s rabies vaccine while other eight were kept as control. Their antibody response wasrnmonitored on 0, 7, 14, 21, 30 and 60 days post vaccination. With regards to potency, thernmedian effective dose (ED50) of the test vaccine was 1.47 while the reference vaccinernwas 1.3. Therefore, relative potency (RP) of the test vaccine found to be 1.45IU/ml inrn1ml of single recommended dose. On the other hand, all dogs recruited forrnimmunological experiment had low antibody titer and the titer of control dogs remainedrnat lower level over experiment period. However, the mean antibody titer of vaccinatedrnpuppies statistically rose to 1.556IU/ml on 7th day of post vaccination. Similarly, thernmean titer increased for the next consecutive two weeks. The antibody titer reached peakrnof 3.585IU/ml on 30th day of post vaccination. Despite of its reduction, it stayed at higherrnlevel until the end of experiment. In general, the test vaccine is potent enough to meetrnrequirement of veterinary inactivated rabies vaccine and is effective and immunogenicrnfor two months of post vaccination. However, immunological experiment in dogs needsrnto be undertaken for longer period and complimented with protective efficacy clinicalrntrial.

Get Full Work

Report copyright infringement or plagiarism